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Title: | Classification of quinoa varieties based on protein fingerprinting by capillary electrophoresis with ultraviolet absorption diode array detection and advanced chemometrics |
Author: | Galindo-Luján, Rocío Pont Villanueva, Laura Sanz Nebot, María Victoria Benavente Moreno, Fernando J. (Julián) |
Keywords: | Proteïnes Cereals (Aliment) Química dels aliments Proteins Grain (feed) Food composition |
Issue Date: | 28-Sep-2020 |
Publisher: | Elsevier B.V. |
Abstract: | Quinoa (Chenopodium quinoa Willd.) is an andean grain with exceptional nutritional properties that has been progressively introduced in western countries as a protein-rich super food with a broad amino acid spectrum. Quinoa is consumed as whole grain, but it is also milled to produce high-value flour, which is susceptible to adulteration. Therefore, there is a growing interest in developing novel analytical methods to get further information about quinoa at the chemical level. In this study, we developed a rapid and simple capillary electrophoresis-ultraviolet absorption diode array detection (CE-UV-DAD) method to obtain characteristic multiwavelength electrophoretic profiles of soluble protein extracts from different quinoa grain varieties. Then, advanced chemometric methods (i.e. multivariate curve resolution alternating least squares, MCR-ALS, followed by principal component analysis, PCA, and partial least squares discriminant analysis, PLS-DA) were applied to deconvolute the components present in the electropherograms and classify the quinoa varieties according to their differential protein composition. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.foodchem.2020.128207 |
It is part of: | Food Chemistry, 2020, vol. 341, p. 128207 |
URI: | http://hdl.handle.net/2445/172184 |
Related resource: | https://doi.org/10.1016/j.foodchem.2020.128207 |
ISSN: | 0308-8146 |
Appears in Collections: | Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB)) Articles publicats en revistes (Enginyeria Química i Química Analítica) |
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