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Title: | Temperature Dependent Control of the R27 Conjugative Plasmid Genes |
Author: | Gibert, Marta Paytubi Casabona, Sònia Madrid Xufré, Cristina Balsalobre Parra, Carlos |
Keywords: | Plasmidis Expressió gènica Plasmids Gene expression |
Issue Date: | 10-Jul-2020 |
Publisher: | Frontiers Media |
Abstract: | Conjugation of R27 plasmid is thermoregulated, being promoted at 25°C and repressed at 37°C. Previous studies identified plasmid-encoded regulators, HtdA, TrhR and TrhY, that control expression of conjugation-related genes (tra). Moreover, the nucleoid-associated protein H-NS represses conjugation at non-permissive temperature. A transcriptomic approach has been used to characterize the effect of temperature on the expression of the 205 R27 genes. Many of the 35 tra genes, directly involved in plasmid-conjugation, were upregulated at 25°C. However, the majority of the non-tra R27 genes many of them with unknown function were more actively expressed at 37°C. The role of HtdA, a regulator that causes repression of the R27 conjugation by counteracting TrhR/TrhY mediated activation of tra genes, has been investigated. Most of the R27 genes are severely derepressed at 25°C in an htdA mutant, suggesting that HtdA is involved also in the repression of R27 genes other than the tra genes. Interestingly, the effect of htdA mutation was abolished at non-permissive temperature, indicating that the HtdA-TrhR/TrhY regulatory circuit mediates the environmental regulation of R27 gene expression. The role of H-NS in the proposed model is discussed. |
Note: | Reproducció del document publicat a: https://doi.org/10.3389/fmolb.2020.00124 |
It is part of: | Frontiers In Molecular Biosciences, 2020, num. 7 |
URI: | http://hdl.handle.net/2445/173522 |
Related resource: | https://doi.org/10.3389/fmolb.2020.00124 |
ISSN: | 2296-889X |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) Articles publicats en revistes (Institut d'lnvestigació Biomèdica de Bellvitge (IDIBELL)) |
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