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Title: Identification of type III secretion inhibitors for plant disease management
Author: Pedro Jové, Roger de
Sebastià, Pau
Valls i Matheu, Marc
Keywords: Plagues agrícoles
Bacteris patògens
Agricultural pests
Pathogenic bacteria
Issue Date: 4-Dec-2020
Publisher: Springer Science + Business Media
Abstract: Bacterial plant pathogens are among the most devastating threats to agriculture. To date, there are no effective means to control bacterial plant diseases due to the restrictions in the use of antibiotics in agriculture. A novel strategy under study is the use of chemical compounds that inhibit the expression of key bacterial virulence determinants. The type III secretion system is essential for virulence of many Gram-negative bacteria because it injects into the plant host cells bacterial proteins that interfere with their immune system. Here, we describe the methodology to identify bacterial type III secretion inhibitors, including a series of protocols that combine in planta and in vitro experiments. We use Ralstonia solanacearum as a model because of the number of genetic tools available in this organism and because it causes bacterial wilt, one of the most threatening plant diseases worldwide. The procedures presented can be used to evaluate the effect of different chemical compounds on bacterial growth and virulence.
Note: Versió postprint del document publicat a:
It is part of: Methods in Molecular Biology, 2020, vol. 2213, p. 39-48
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ISSN: 1940-6029
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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