Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/184139
Title: | Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage |
Author: | Itarte, Marta Bofill Mas, Silvia Martínez-Puchol, Sandra Torrell, Helena Ceretó, Adrià Carrasco, Marina (Carrasco Pérez) Forés, Eva Canela, Núria Gironès Llop, Rosina Rusiñol, Marta |
Keywords: | SARS-CoV-2 Aigües residuals SARS-CoV-2 Sewage |
Issue Date: | Dec-2021 |
Publisher: | Elsevier B.V. |
Abstract: | SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1016/j.coesh.2021.100308 |
It is part of: | Current Opinion in Environmental Science & Health, 2021, vol. 24, p. 100308 |
URI: | http://hdl.handle.net/2445/184139 |
Related resource: | https://doi.org/10.1016/j.coesh.2021.100308 |
ISSN: | 2468-5844 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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720366.pdf | 2.67 MB | Adobe PDF | View/Open |
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