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Title: Looking for a needle in a haystack. SARS-CoV-2 variant characterization in sewage
Author: Itarte, Marta
Bofill Mas, Silvia
Martínez-Puchol, Sandra
Torrell, Helena
Ceretó, Adrià
Carrasco, Marina (Carrasco Pérez)
Forés, Eva
Canela, Núria
Gironès Llop, Rosina
Rusiñol, Marta
Keywords: SARS-CoV-2
Aigües residuals
Issue Date: Dec-2021
Publisher: Elsevier B.V.
Abstract: SARS-CoV-2 variants are emerging worldwide, and monitoring them is key in providing early warnings. Here, we summarize the different analytical approaches currently used to study the dissemination of SARS-CoV-2 variants in wastewater and discuss their advantages and disadvantages. We also provide preliminary results of two sensitive and cost-effective approaches: variant-specific reverse transcription-nested PCR assays and a nonvariant-specific amplicon deep sequencing strategy that targets three key regions of the viral spike protein. Next-generation sequencing approaches enable the simultaneous detection of signature mutations of different variants of concern in a single assay and may be the best option to explore the real picture at a particular time. Targeted PCR approaches focused on specific signature mutations will need continuous updating but are sensitive and cost-effective.
Note: Versió postprint del document publicat a:
It is part of: Current Opinion in Environmental Science & Health, 2021, vol. 24, p. 100308
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ISSN: 2468-5844
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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