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Title: A new variant of the addE-sat4-aphA-3 gene cluster found in a conjugative plasmid from a MDR Campylobacter jejuni isolate.
Author: Guirado, Pedro
Miró, Elisenda
Iglesias-Torrens, Yaidelis
Navarro, Ferran
Campoy, Susana
Alioto, Tyler Scott
Gómez-Garrido, Jessica
Madrid Xufré, Cristina
Balsalobre Parra, Carlos
Keywords: Toxicologia alimentària
Resistència als medicaments
Food toxicology
Drug resistance
Issue Date: 30-Mar-2022
Publisher: MDPI
Abstract: Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera.
Note: Reproducció del document publicat a:
It is part of: Antibiotics, 2022, vol. 11, num. 4, p. 1-14
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ISSN: 2079-6382
Appears in Collections:Articles publicats en revistes (Genètica, Microbiologia i Estadística)

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