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Title: | A new variant of the addE-sat4-aphA-3 gene cluster found in a conjugative plasmid from a MDR Campylobacter jejuni isolate. |
Author: | Guirado, Pedro Miró, Elisenda Iglesias-Torrens, Yaidelis Navarro, Ferran Campoy, Susana Alioto, Tyler Scott Gómez-Garrido, Jessica Madrid Xufré, Cristina Balsalobre Parra, Carlos |
Keywords: | Toxicologia alimentària Resistència als medicaments Antibiòtics Gastroenteritis Food toxicology Drug resistance Antibiotics Gastroenteritis |
Issue Date: | 30-Mar-2022 |
Publisher: | MDPI |
Abstract: | Campylobacter jejuni is a foodborne pathogen causing bacterial gastroenteritis, with the highest incidence reported in Europe. The prevalence of antibiotic resistance in C. jejuni, as well as in many other bacterial pathogens, has increased over the last few years. In this report, we describe the presence of a plasmid in a multi-drug-resistant C. jejuni strain isolated from a gastroenteritis patient. Mating experiments demonstrated the transference of this genetic element (pCjH01) among C. jejuni by plasmid conjugation. The pCjH01 plasmid was sequenced and assembled, revealing high similarity (97% identity) with pTet, a described tetracycline resistance encoding plasmid. pCjH01 (47.7 kb) is a mosaic plasmid composed of a pTet backbone that has acquired two discrete DNA regions. Remarkably, one of the acquired sequences carried an undescribed variant of the aadE-sat4-aphA-3 gene cluster, providing resistance to at least kanamycin and gentamycin. Aside from the antibiotic resistance genes, the cluster also carries genes coding for putative regulators, such as a sigma factor of the RNA polymerase and an antisigma factor. Homology searches suggest that Campylobacter exchanges genetic material with distant G-positive bacterial genera. |
Note: | Reproducció del document publicat a: https://doi.org/10.3390/antibiotics11040466 |
It is part of: | Antibiotics, 2022, vol. 11, num. 4, p. 1-14 |
URI: | http://hdl.handle.net/2445/184543 |
Related resource: | https://doi.org/10.3390/antibiotics11040466 |
ISSN: | 2079-6382 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
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