Please use this identifier to cite or link to this item: http://hdl.handle.net/2445/191944
Title: Antimicrobial and Photoantimicrobial Activities of Chitosan/CNPPV Nanocomposites
Author: Facchinatto, William M.
Araujo, Leandro O.
Moraes, Tiago B.
Abelha, Thais F.
Lima, Thalita H. N.
Dos Santos, Danilo M.
Campana-Filho, Sérgio P.
Colnago, Luiz A.
Caires, Anderson R. L.
Keywords: Nanopartícules
Quitosan
Microbiologia
Nanoparticles
Chitosan
Microbiology
Issue Date: 19-Oct-2022
Publisher: MDPI
Abstract: Multidrug-resistant bacteria represent a global health and economic burden that urgently calls for new technologies to combat bacterial antimicrobial resistance. Here, we developed novel nanocomposites (NCPs) based on chitosan that display different degrees of acetylation (DAs), and conjugated polymer cyano-substituted poly(p-phenylene vinylene) (CNPPV) as an alternative approach to inactivate Gram-negative (E. coli) and Gram-positive (S. aureus) bacteria. Chitosan's structure was confirmed through FT-Raman spectroscopy. Bactericidal and photobactericidal activities of NCPs were tested under dark and blue-light irradiation conditions, respectively. Hydrodynamic size and aqueous stability were determined by DLS, zeta potential (ZP) and time-domain NMR. TEM micrographs of NCPs were obtained, and their capacity of generating reactive oxygen species (ROS) under blue illumination was also characterized. Meaningful variations on ZP and relaxation time T2 confirmed successful physical attachment of chitosan/CNPPV. All NCPs exhibited a similar and shrunken spherical shape according to TEM. A lower DA is responsible for driving higher bactericidal performance alongside the synergistic effect from CNPPV, lower nanosized distribution profile and higher positive charged surface. ROS production was proportionally found in NCPs with and without CNPPV by decreasing the DA, leading to a remarkable photobactericidal effect under blue-light irradiation. Overall, our findings indicate that chitosan/CNPPV NCPs may constitute a valuable asset for the development of innovative strategies for inactivation and/or photoinactivation of bacteria. Keywords: photoantimicrobial activity; blue-light irradiation; chitosan; CNPPV; nanocomposites; E. coli; S. aureus
Note: Reproducció del document publicat a: https://doi.org/10.3390/ijms232012519
It is part of: International Journal of Molecular Sciences, 2022, vol. 23, p. 12519
URI: http://hdl.handle.net/2445/191944
Related resource: https://doi.org/10.3390/ijms232012519
ISSN: 1661-6596
Appears in Collections:Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

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