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http://hdl.handle.net/2445/192215
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DC Field | Value | Language |
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dc.contributor.author | Letelier, Joaquín | - |
dc.contributor.author | De La Calle-Mustienes, Elisa | - |
dc.contributor.author | Pieretti, Joyce | - |
dc.contributor.author | Naranjo, Silvia | - |
dc.contributor.author | Maeso, Ignacio | - |
dc.contributor.author | Nakamura, Tetsuya | - |
dc.contributor.author | Pascual-Anaya, Juan | - |
dc.contributor.author | Shubin, Neil H | - |
dc.contributor.author | Igor, Schneider | - |
dc.contributor.author | Martinez-Morales, Juan Ramón | - |
dc.contributor.author | Gómez-Skarmeta, José Luis | - |
dc.date.accessioned | 2023-01-17T11:00:53Z | - |
dc.date.available | 2023-01-17T11:00:53Z | - |
dc.date.issued | 2018 | - |
dc.identifier.issn | 1061-4036 | - |
dc.identifier.uri | http://hdl.handle.net/2445/192215 | - |
dc.description.abstract | Despite their evolutionary, developmental, and functional importance the origin of vertebrate paired appendages remains uncertain. In mice, a single enhancer termed ZRS is solely responsible for Shh expression in limbs. Here, zebrafish and mouse transgenic assays trace the functional equivalence of ZRS across the gnathostome phylogeny. CRISPR/Cas9-mediated deletion of the medaka-ZRS and enhancer assays reveal the existence of ZRS shadow enhancers in both teleost and human genomes. Deletion of both ZRS and shadow ZRS abolish shh expression and completely truncate pectoral fin formation. Strikingly, deletion of ZRS results in an almost complete ablation of the dorsal fin. This finding indicates that a ZRS-Shh regulatory module is shared by paired and median fins, and that paired fins likely emerged by the co‐option of developmental programs established in the median fins of stem gnathostomes. Shh function was later reinforced in pectoral fin development with the recruitment of shadow enhancers, conferring additional robustness. | - |
dc.format.extent | 6 p. | - |
dc.format.mimetype | application/pdf | - |
dc.language.iso | eng | - |
dc.publisher | Nature Publishing Group | - |
dc.relation.isformatof | Versió postprint del document publicat a: https://doi.org/10.1038/s41588-018-0080-5 | - |
dc.relation.ispartof | Nature Genetics, 2018, vol. 50, num. 4, p. 504-509 | - |
dc.relation.uri | https://doi.org/10.1038/s41588-018-0080-5 | - |
dc.rights | (c) Letelier, Joaquín et al., 2018 | - |
dc.source | Articles publicats en revistes (Genètica, Microbiologia i Estadística) | - |
dc.subject.classification | Vertebrats | - |
dc.subject.classification | Gens | - |
dc.subject.classification | Genoma humà | - |
dc.subject.other | Vertebrates | - |
dc.subject.other | Genes | - |
dc.subject.other | Human genome | - |
dc.title | A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins | - |
dc.type | info:eu-repo/semantics/article | - |
dc.type | info:eu-repo/semantics/acceptedVersion | - |
dc.identifier.idgrec | 722956 | - |
dc.date.updated | 2023-01-17T11:00:53Z | - |
dc.rights.accessRights | info:eu-repo/semantics/openAccess | - |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
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722956.pdf | 1.94 MB | Adobe PDF | View/Open |
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