Please use this identifier to cite or link to this item:
http://hdl.handle.net/2445/192215
Title: | A conserved Shh cis-regulatory module highlights a common developmental origin of unpaired and paired fins |
Author: | Letelier, Joaquín De La Calle-Mustienes, Elisa Pieretti, Joyce Naranjo, Silvia Maeso, Ignacio Nakamura, Tetsuya Pascual-Anaya, Juan Shubin, Neil H Igor, Schneider Martinez-Morales, Juan Ramón Gómez-Skarmeta, José Luis |
Keywords: | Vertebrats Gens Genoma humà Vertebrates Genes Human genome |
Issue Date: | 2018 |
Publisher: | Nature Publishing Group |
Abstract: | Despite their evolutionary, developmental, and functional importance the origin of vertebrate paired appendages remains uncertain. In mice, a single enhancer termed ZRS is solely responsible for Shh expression in limbs. Here, zebrafish and mouse transgenic assays trace the functional equivalence of ZRS across the gnathostome phylogeny. CRISPR/Cas9-mediated deletion of the medaka-ZRS and enhancer assays reveal the existence of ZRS shadow enhancers in both teleost and human genomes. Deletion of both ZRS and shadow ZRS abolish shh expression and completely truncate pectoral fin formation. Strikingly, deletion of ZRS results in an almost complete ablation of the dorsal fin. This finding indicates that a ZRS-Shh regulatory module is shared by paired and median fins, and that paired fins likely emerged by the co‐option of developmental programs established in the median fins of stem gnathostomes. Shh function was later reinforced in pectoral fin development with the recruitment of shadow enhancers, conferring additional robustness. |
Note: | Versió postprint del document publicat a: https://doi.org/10.1038/s41588-018-0080-5 |
It is part of: | Nature Genetics, 2018, vol. 50, num. 4, p. 504-509 |
URI: | http://hdl.handle.net/2445/192215 |
Related resource: | https://doi.org/10.1038/s41588-018-0080-5 |
ISSN: | 1061-4036 |
Appears in Collections: | Articles publicats en revistes (Genètica, Microbiologia i Estadística) |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
722956.pdf | 1.94 MB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.