Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/231982
From mold to mill: StachCel5, a novel thermoalkaliphilic endoglucanase from Stachybotrys chartarum for pulp fiber biorefining
Títol de la revista
Director/Tutor
Contribució addicional
ISSN de la revista
Títol del volum
Resum
StachCel5, a novel endo-β-1,4-glucanase from Stachybotrys chartarum IBT 7711, was identified through genome mining and exhibited 65 % sequence identity with known glycoside hydrolase family 5 (GH5) cellulases. The gene was codon-optimized, excluding its native signal peptide, and heterologously expressed in Komagataella phaffii under the control of constitutive glyceraldehye-3-phosphate dehydrogenase (GAP) promoter. The purified enzyme displayed a high specific activity of 287.6 U/mg on carboxymethyl cellulose (Na-CMC), retained over 87 % activity across pH 4–7, and maintained 77 % residual activity after 60 min at 50 °C. Structural modeling predicted a canonical (β/α)₈ TIM-barrel fold, with Glu228 and Glu335 as conserved catalytic residues. Application trials on industrial hardwood and softwood pulps demonstrated that enzymatic pre-treatment with StachCel5 enhanced fiber fibrillation, reduced refining energy, and improved mechanical properties. In softwood pulp, the enzyme enabled equivalent tensile strength (67.8 Nm/g) as untreated controls with 25 % fewer refining revolutions (4500 vs 6000), reflecting significant energy savings. Improved tensile and burst indices, and preserved fiber integrity (zero-span tensile index) confirm the biorefining potential of StachCel5. These results establish StachCel5 as a thermostable and pH-tolerant biocatalyst suitable for enzymatic fiber modification in pulp and paper processing under industrially relevant conditions.
Recurs relacionat
Matèries
Matèries (anglès)
Citació
Citació
FERRANDO NÚÑEZ, Jordi, et al. From mold to mill: StachCel5, a novel thermoalkaliphilic endoglucanase from Stachybotrys chartarum for pulp fiber biorefining. International Journal of Biological Macromolecules. 2025. Vol. 320, num. 3. ISSN 0141-8130. [consulted: 11 of November of 2026]. Available at: https://hdl.handle.net/2445/231982