Dijous 11 de juny, el Dipòsit Digital no estarà operatiu de 15:00 a 17:00 h per tasques de manteniment. Disculpeu les molèsties.
El jueves 11 de Junio, el Dipòsit Digital no estará operativo de 15:00 a 17:00 h debido a tareas de mantenimiento. Disculpen las molestias.
Thursday, Jun 11th, the Digital Repository will be unavailable due to a system update.

Files

mari-almirall2016_2326.pdf (1.77 MB)

Document type

Article

Version

Accepted version

Publication date

2016-12-02

Publication license

cc by-nc-nd (c) Elsevier, 2016
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/105105

MALDI-TOF/MS identification of species from the Acinetobacter baumannii (Ab) group revisited: inclusion of the novel A. seifertii and A. dijkshoorniae species

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

http://dx.doi.org/10.1016/j.cmi.2016.11.020

Abstract

OBJECTIVES: Rapid identification of Acinetobacter species is critical since members of the A. baumannii (Ab) group differ in antibiotic susceptibility and clinical outcomes. A. baumannii, A. pittii and A. nosocomialis can be identified by MALDI-TOF/MS, while the novel species A. seifertii and A. dijkshoorniae cannot. Low identification rates for A. nosocomialis have also been reported. We evaluated the use of MALDI-TOF/MS to identify isolates of A. seifertii and A. dijkshoorniae and revisited the identification of A. nosocomialis to update the Bruker taxonomy database. METHODS: Species characterisation was performed by rpoB-clustering and MLSA. MALDI-TOF/MS spectra were recovered from formic acid/acetonitrile bacterial extracts overlaid with alpha-cyano-4-hydroxy-cinnamic acid matrix on a MicroflexLT in linear positive mode and 2,000-20,000 m/z range mass. Spectra were examined with the ClinProTools v2.2 software. Mean spectra (MSP) were created with the BioTyper software. RESULTS: Seventy-eight Acinetobacter isolates representative of the Ab group were used to calculate the average spectra/species and generate pattern recognition models. Species-specific peaks were identified for all species, and MSPs derived from 3 A. seifertii, 2 A. dijkshoorniae and 2 A. nosocomialis strains were added to the Bruker taxonomy database, allowing successful identification of all isolates using spectra from either bacterial extracts or direct colonies, resulting in a positive predictive value (PPV) of 99.6% (777/780) and 96.8% (302/312), respectively. CONCLUSIONS: The use of post-processing data software identified statistically significant species-specific peaks to generate reference signatures for rapid accurate identification of species within the Ab group, providing relevant information for the clinical management of Acinetobacter infections.

Subject

Bacteris gramnegatius, Resistència als medicaments

Subject (English)

Gram-negative bacteria, Drug resistance

Citation

Collections

Articles publicats en revistes (ISGlobal)

Citation

MARÍ ALMIRALL, Marta, et al. MALDI-TOF/MS identification of species from the Acinetobacter
                baumannii (Ab) group revisited: inclusion of the novel A.
                seifertii and A. dijkshoorniae species. Clinical Microbiology and Infection. 2016. ISSN 1198-743X. [consulted: 11 of June of 2026]. Available at: https://hdl.handle.net/2445/105105

Export metadata

JSON - METS

Share record