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2012-03-28

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cc-by (c) Sancho, Ana et al., 2012
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/124627

DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription

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https://doi.org/10.1371/journal.pone.0034034

Resum

Human DOR/TP53INP2 displays a unique bifunctional role as a modulator of autophagy and gene transcription. However, the domains or regions of DOR that participate in those functions have not been identified. Here we have performed structure/function analyses of DOR guided by identification of conserved regions in the DOR gene family by phylogenetic reconstructions. We show that DOR is present in metazoan species. Invertebrates harbor only one gene, DOR/Tp53inp2, and in the common ancestor of vertebrates Tp53inp1 may have arisen by gene duplication. In keeping with these data, we show that human TP53INP1 regulates autophagy and that different DOR/TP53INP2 and TP53INP1 proteins display transcriptional activity. The use of molecular evolutionary information has been instrumental to determine the regions that participate in DOR functions. DOR and TP53INP1 proteins share two highly conserved regions (region 1, aa residues 28-42; region 2, 66-112 in human DOR). Mutation of conserved hydrophobic residues in region 1 of DOR (that are part of a nuclear export signal, NES) reduces transcriptional activity, and blocks nuclear exit and autophagic activity under autophagy-activated conditions. We also identify a functional and conserved LC3-interacting motif (LIR) in region 1 of DOR and TP53INP1 proteins. Mutation of conserved acidic residues in region 2 of DOR reduces transcriptional activity, impairs nuclear exit in response to autophagy activation, and disrupts autophagy. Taken together, our data reveal DOR and TP53INP1 as dual regulators of transcription and autophagy, and identify two conserved regions in the DOR family that concentrate multiple functions crucial for autophagy and transcription.

Matèries

Autofàgia, Transcripció genètica

Matèries (anglès)

Autophagy, Genetic transcription

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

SANCHO MEDINA, Ana, DURAN, Jordi, GARCÍA-ESPAÑA, Antonio, MAUVEZIN, Caroline, ALEMU, Endalkachew a., LAMARK, Trond, MACÍAS HERNÁNDEZ, María j., DESALLE, Rob, ROYO EXPÓSITO, Miriam, SALA CANO, David, CHICOTE, Javier u., PALACÍN PRIETO, Manuel, JOHANSEN, Terje, ZORZANO OLARTE, Antonio. DOR/Tp53inp2 and Tp53inp1 constitute a metazoan gene family encoding dual regulators of autophagy and transcription. _PLoS One_. 2012. Vol. 7, núm. 3, pàgs. 1-19. [consulta: 1 de febrer de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/124627]

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