Parallel Illumination in Super-resolution Microscopy with Photoswitchable Fluorophores

Abstract

This work demonstrates a parallelized scanning RESOLFT microscope using Acousto-Optic Deflectors to generate co-aligned matrix patterns, offering a cost-effective, solid-state approachto superresolution nanoscopy. The system integrates 405 nm activation, 488 nm readout and donutshapeddepletion beams, enabling rapid, inertialess scanning through programmable phase modulation. While technical challenges related to stability and laser performance prevented replicable sub-diffraction imaging, the setup achieved high-contrast parallelized confocal imaging, validating the underlying optical path and establishing a baseline for accessible superresolution microscopy