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2017-12-18

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cc-by-nc-nd (c) Elsevier B.V., 2017
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/143335

Molecular interactions between warfarin and human (HSA) or bovine(BSA) serum albumin evaluated by isothermal titration calorimetry(ITC), fluorescence spectrometry (FS) and frontal analysis capillaryelectrophoresis (FA/CE)

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https://doi.org/10.1016/j.jpba.2017.12.008

Resum

Interaction thermodynamics between warfarin, a very popular anticoagulant, and Sudlow I binding siteof human (HSA) or bovine (BSA) serum albumin have been examined in strictly controlled experimentalconditions (HEPES buffer 50 mM, pH 7.4 and 25◦C) by means of isothermal titration calorimetry (ITC),fluorescence spectrometry (FS) and frontal analysis capillary electrophoresis (FA/CE). Each technique isbased on measurements of a different property of the biochemical system, and then the results allow acritical discussion about the suitability of each approach to estimate the drug-protein binding parameters.The strongest interaction step is properly evaluated by the three assayed approaches being the derivedbinding constants strongly consistent: from 4 × 104to 7 × 104for HSA and from 0.8 × 105to 1.2 × 105forBSA. Binding enthalpy variations also show consistent results: −5.4 and −5.6 Kcal/mol for HSA and −4.3and −3.7 Kcal/mol for BSA, as measured by ITC and FS, respectively. Further high order interaction eventsfor both albumins are detected only by FA/CE.

Matèries

Anticoagulants (Medicina), Electroforesi capil·lar

Matèries (anglès)

Anticoagulants (Medicine), Capillary electrophoresis

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Articles publicats en revistes (Enginyeria Química i Química Analítica)

Citació

RÀFOLS LLACH, Clara, et al. Molecular interactions between warfarin and human (HSA) or bovine(BSA) serum albumin evaluated by isothermal titration calorimetry(ITC), fluorescence spectrometry (FS) and frontal analysis capillaryelectrophoresis (FA/CE). Journal of Pharmaceutical and Biomedical Analysis. 2017. Vol. 150, num. 452-459. ISSN 0731-7085. [consulted: 22 of June of 2026]. Available at: https://hdl.handle.net/2445/143335

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