Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/194422
S-acylation-dependent membrane microdomain localization of the regulatory Kvβ2.1 subunit
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The voltage-dependent potassium (Kv) channel Kvβ family was the frst identifed group of modulators of Kv channels. Kvβ regulation of the α-subunits, in addition to their aldoketoreductase activity, has been under extensive study. However, scarce information about their specifc α-subunit-independent biology is available. The expression of Kvβs is ubiquitous and, similar to Kv channels, is tightly regulated in leukocytes. Although Kvβ subunits exhibit cytosolic distribution, spatial localization, in close contact with plasma membrane Kv channels, is crucial for a proper immune response. Therefore, Kvβ2.1 is located near cell surface Kv1.3 channels within the immunological synapse during lymphocyte activation. The objective of this study was to analyze the structural elements that participate in the cellular distribution of Kvβs. It was demonstrated that Kvβ peptides, in addition to the cytoplasmic pattern, targeted the cell surface in the absence of Kv channels. Furthermore, Kvβ2.1, but not Kvβ1.1, targeted lipid raft microdomains in an S-acylation-dependent manner, which was concomitant with peptide localization within the immunological synapse. A pair of C-terminal cysteines (C301/C311) was mostly responsible for the specifc palmitoylation of Kvβ2.1. Several insults altered Kvβ2.1 membrane localization. Therefore, growth factor-dependent proliferation enhanced surface targeting, whereas PKC activation impaired lipid raft expression. However, PSD95 stabilized Kvβ2.1 in these domains. This data shed light on the molecular mechanism by which Kvβ2.1 clusters into immunological synapses during leukocyte activation.
Matèries
Matèries (anglès)
Citació
Citació
ROIG, Sara r., CASSINELLI, Silvia, NAVARRO-PÉREZ, María, PÉREZ-VERDAGUER, Mireia, ESTADELLA, Irene, CAPERA ARAGONÉS, Jesusa, FELIPE CAMPO, Antonio. S-acylation-dependent membrane microdomain localization of the regulatory Kvβ2.1 subunit. _Cellular and Molecular Life Sciences_. 2022. Vol. 79, núm. 5, pàgs. 230. [consulta: 21 de gener de 2026]. ISSN: 1420-682X. [Disponible a: https://hdl.handle.net/2445/194422]