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2018-11-28

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cc-by (c) Ugarte, Laura de et al., 2018
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/130348

Pro-osteoporotic miR-320a impairs osteoblast function and induces oxidative stress

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https://doi.org/10.1371/journal.pone.0208131

Resum

MicroRNAs (miRNAs) are important regulators of many cellular processes, including the differentiation and activity of osteoblasts, and therefore, of bone turnover. MiR-320a is overexpressed in osteoporotic bone tissue but its role in osteoblast function is unknown. In the present study, functional assays were performed with the aim to elucidate the mechanism of miR-320a action in osteoblastic cells. MiR-320a was either overexpressed or inhibited in human primary osteoblasts (hOB) and gene expression changes were evaluated through microarray analysis. In addition, the effect of miR-320a on cell proliferation, viability, and oxidative stress in hOB was evaluated. Finally, matrix mineralization and alkaline phosphatase activity were assessed in order to evaluate osteoblast functionality. Microarray results showed miR-320a regulation of a number of key osteoblast genes and of genes involved in oxidative stress. Regulation of osteoblast differentiation and ossification appeared as the best significant biological processes (PANTHER P value=3.74E-05; and P value=3.06E-04, respectively). The other enriched pathway was that of the cellular response to cadmium and zinc ions, mostly by the overexpression of metallothioneins. In hOBs, overexpression of miR-320a increased cell proliferation and oxidative stress levels whereas mineralization capacity was reduced. In conclusion, overexpression of miR-320a increased stress oxidation levels and was associated with reduced osteoblast differentiation and functionality, which could trigger an osteoporotic phenotype.

Matèries

Expressió gènica, Estrès oxidatiu, Osteoporosi, Teixit ossi

Matèries (anglès)

Gene expression, Oxidative stress, Osteoporosis, Bone

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Col·leccions

Articles publicats en revistes (Genètica, Microbiologia i Estadística)
Articles publicats en revistes (Institut de Biomedicina (IBUB))

Citació

UGARTE, Laura de, et al. Pro-osteoporotic miR-320a impairs osteoblast function and induces oxidative stress. PLoS One. 2018. Vol. 13, núm. 11, pàgs. e0208131. ISSN 1932-6203. [consulta: 8 de maig de 2026]. Disponible a: https://hdl.handle.net/2445/130348

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