Miniatura

Fitxers

662614.pdf (1018.87 KB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2016-05-31

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/165883

Effects of dietary carbohydrate on hepatic de novo lipogenesis in European seabass (Dicentrarchus labrax L.)

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1194/jlr.M067850

Resum

Farmed seabass have higher adiposity than their wild counterparts and this is often attributed to carbohydrate (CHO) feeding. Whether this reflects a reduction in fat oxidation, increased de novo lipogenesis (DNL), or both, is not known. To study the effects of high CHO diets on hepatic TG biosynthesis, hepatic TG deuterium (2H) enrichment was determined following 6 days in 2H-enriched tank water for fish fed with a no-CHO control diet (CTRL), and diets with digestible starch (DS) and raw starch (RS). Hepatic fractional synthetic rates (FSRs, percent per day-1) were calculated for hepatic TG-glyceryl and FA moieties through 2H NMR analysis. Glyceryl FSRs exceeded FA FSRs in all cases, indicating active cycling. DS fish did not show increased lipogenic potential compared to CTRL. RS fish had lower glyceryl FSRs compared with the other diets and negligible levels of FA FSRs despite similar hepatic TG levels to CTRL. DS-fed fish showed higher activity for enzymes that can provide NADPH for lipogenesis, relative to CTRL in the case of glucose-6-phosphate dehydrogenase (G6PDH) and relative to RS for both G6PDH and 6-phosphogluconate dehydrogenase. This approach indicated that elevated hepatic adiposity from DS feeding was not attributable to increased DNL.

Matèries

Fisiologia, Metabolisme, Deshidrogenases, Aqüicultura, Nutrició, Fetge, Àcids grassos, Glúcids

Matèries (anglès)

Physiology, Metabolism, Dehydrogenases, Aquaculture, Nutrition, Liver, Fatty acids, Glucides

Citació

Col·leccions

Articles publicats en revistes (Bioquímica i Fisiologia)

Citació

VIEGAS, Iván, JARAK, Ivana, RITO, Joao, CARVALHO, Rui a., METÓN TEIJEIRO, Isidoro, PARDAL, Miguel a., BAANANTE, Isabel v., JONES, John g.. Effects of dietary carbohydrate on hepatic de novo lipogenesis in European seabass (Dicentrarchus labrax L.). _Journal of Lipid Research_. 2016. Vol. 57, núm. 7, pàgs. 1264-1272. [consulta: 23 de gener de 2026]. ISSN: 0022-2275. [Disponible a: https://hdl.handle.net/2445/165883]

Exportar metadades

JSON - METS

Compartir registre