Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/159422
Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
In vitro surrogate models of human cardiac tissue hold great promise in disease modeling, cardiotoxicity testing, and future applications in regenerative medicine. However, the generation of engineered human cardiac constructs with tissue-like functionality is currently thwarted by difficulties in achieving efficient maturation at the cellular and/or tissular level. Here, we report on the design and implementation of a platform for the production of engineered cardiac macrotissues from human pluripotent stem cells (PSCs), which we term "CardioSlice." PSC-derived cardiomyocytes, together with human fibroblasts, are seeded into large 3D porous scaffolds and cultured using a parallelized perfusion bioreactor with custom-made culture chambers. Continuous electrical stimulation for 2 weeks promotes cardiomyocyte alignment and synchronization, and the emergence of cardiac tissue-like properties. These include electrocardiogram-like signals that can be readily measured on the surface of CardioSlice constructs, and a response to proarrhythmic drugs that is predictive of their effect in human patients.
Matèries
Matèries (anglès)
Citació
Citació
VALLS MARGARIT, Maria, IGLESIAS GARCÍA, Olalla, GUGLIELMO, Claudia di, SARLABOUS, Leonardo, TADEVOSYAN, Karine, PAOLI, Roberto, COMELLES PUJADAS, Jordi, BLANCO ALMAZÁN, Dolores, JIMÉNEZ DELGADO, Senda, CASTILLO FERNÁNDEZ, Óscar, SAMITIER I MARTÍ, Josep, JANÉ, Raimon, MARTÍNEZ FRAIZ, Elena, RAYA CHAMORRO, Ángel. Engineered Macroscale Cardiac Constructs Elicit Human Myocardial Tissue-like Functionality. _Stem Cell Reports_. 2019. Vol. 13, núm. 1, pàgs. 207-220. [consulta: 7 de febrer de 2026]. ISSN: 2213-6711. [Disponible a: https://hdl.handle.net/2445/159422]