Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/198767
Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
Topographical patterns are a powerful tool to study directional migration. Grooved substrates have been extensively used as in vitro models of aligned extracellular matrix fibers because they induce cell elongation, alignment, and migration through a phenomenon known as contact guidance. This process, which involves the orientation of focal adhesions, F-actin, and microtubule cytoskeleton along the direction of the grooves, has been primarily studied on hard materials of non-physiological stiffness. But how it unfolds when the stiffness of the grooves varies within the physiological range is less known. Here we show that substrate stiffness modulates the cellular response to topographical contact guidance. We find that for fibroblasts, while focal adhesions and actin respond to topography independently of the stiffness, microtubules show a stiffness-dependent response that regulates contact guidance. On the other hand, both clusters and single breast carcinoma epithelial cells display stiffnessdependent contact guidance, leading to more directional and efficient migration when increasing substrate stiffness. These results suggest that both matrix stiffening and alignment of extracellular matrix fibers cooperate during directional cell migration, and that the outcome differs between cell types depending on how they organize their cytoskeletons.
Matèries
Matèries (anglès)
Citació
Citació
COMELLES PUJADAS, Jordi, FERNÁNDEZ MAJADA, Vanesa, ACEVEDO, Verónica, REBOLLO CALDERÓN, Beatriz, MARTÍNEZ FRAIZ, Elena. Soft topographical patterns trigger a stiffness-dependent cellular response to contact guidance. _Materials Today Bio_. 2022. Vol. 19, núm. 100593. [consulta: 23 de gener de 2026]. ISSN: 2590-0064. [Disponible a: https://hdl.handle.net/2445/198767]