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520442.pdf (461.72 KB)

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2001

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cc-by, (c) Valero et al., 2001
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/7381

Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25

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Recurs relacionat

http://dx.doi.org/10.1186/gb-2001-2-10-research0043

Resum

Background: The ubiquitin-dependent protein degradation pathway is essential for the proteolysis of intracellular proteins and peptides. Deubiquitinating enzymes constitute a complex protein family involved in a multitude of cellular processes. The ubiquitin-specific proteases (UBP) are a group of enzymes whose predicted function is to reverse the ubiquitinating reaction by removing ubiquitin from a large variety of substrates. We have lately reported the characterization of human USP25, a specific-ubiquitin protease gene at 21q11.2, with a specific pattern of expression in murine fetal brains and adult testis. Results: Database homology searches at the DNA and protein levels and cDNA library screenings led to the identification of a new UBP member in the human genome, named USP28, at 11q23. This novel gene showed preferential expression in heart and muscle. Moreover, cDNA, expressed sequence tag and RT-PCR analyses provided evidence for alternatively spliced products and tissue-specific isoforms. Concerning function, USP25 overexpression in Down syndrome fetal brains was shown by real-time PCR. Conclusions: On the basis of the genomic and protein sequence as well as the functional data, USP28 and USP25 establish a new subfamily of deubiquitinating enzymes. Both genes have alternatively spliced exons that could generate protein isoforms with distinct tissue-specific activity. The overexpression of USP25 in Down syndrome fetal brains supports the gene-dosage effects suggested for other UBP members related to aneuploidy syndromes.

Matèries

Biologia cel·lular, Genètica molecular, Medicina, Neurobiologia

Matèries (anglès)

Cell biology, Molecular biology, Medicine, Neurobiology

Citació

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citació

VALERO GILS, Rebeca, et al. Characterization of alternatively spliced products and tissue-specific isoforms of USP28 and USP25. Genome Biology. 2001. Vol. 2, num. 10. ISSN 1465-6914. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/7381

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