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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/61172
Functional Identification of Proteus mirabilis eptC gene encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase
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By comparison of the Proteus mirabilis HI4320 genome with known lipopolysaccharide (LPS) phosphoethanolamine transferases, three putative candidates (PMI3040, PMI3576, and PMI3104) were identified. One of them, eptC (PMI3104) was able to modify the LPS of two defined non-polar core LPS mutants of Klebsiella pneumoniae that we use as surrogate substrates. Mass spectrometry and nuclear magnetic resonance showed that eptC directs the incorporation of phosphoethanolamine to the O-6 of l-glycero-d-mano-heptose II. The eptC gene is found in all the P. mirabilis strains analyzed in this study. Putative eptC homologues were found for only two additional genera of the Enterobacteriaceae family, Photobacterium and Providencia. The data obtained in this work supports the role of the eptC (PMI3104) product in the transfer of PEtN to the O-6 of l,d-HepII in P. mirabilis strains.
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AQUILINI, Eleonora, et al. Functional Identification of Proteus mirabilis eptC gene encoding a Core Lipopolysaccharide Phosphoethanolamine Transferase. International Journal of Molecular Sciences. 2014. Vol. 15, num. 6689-6702. ISSN 1422-0067. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/61172