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12541_6467667_savatier_stemcellrep_2021.pdf (8.12 MB)

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2021-01-12

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cc by (c) Aksoy, Irène et al., 2021
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/174577

Apoptosis, G1 Phase Stall, and Premature Differentiation Account for Low Chimeric Competence of Human and Rhesus Monkey Naive Pluripotent Stem Cells

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https://doi.org/10.1016/j.stemcr.2020.12.004

Resum

After reprogramming to naive pluripotency, human pluripotent stem cells (PSCs) still exhibit very low ability to make interspecies chimeras. Whether this is because they are inherently devoid of the attributes of chimeric competency or because naive PSCs cannot colonize embryos from distant species remains to be elucidated. Here, we have used different types of mouse, human, and rhesus monkey naive PSCs and analyzed their ability to colonize rabbit and cynomolgus monkey embryos. Mouse embryonic stem cells (ESCs) remained mitotically active and efficiently colonized host embryos. In contrast, primate naive PSCs colonized host embryos with much lower efficiency. Unlike mouse ESCs, they slowed DNA replication after dissociation and, after injection into host embryos, they stalled in the G1 phase and differentiated prematurely, regardless of host species. We conclude that human and non-human primate naive PSCs do not efficiently make chimeras because they are inherently unfit to remain mitotically active during colonization.

Matèries

Cèl·lules mare, Mones, Éssers humans

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Stem cells, Monkeys, Human beings

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Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citació

AKSOY, Irène, AFANASSIEFF, Marielle, BELLEMIN MÉNARD, Angèle, BOURILLOT, Pierre yves, CORTAY, Véronique, DEHAY, Colette, DIRHEIMER, Manon, DOERFLINGER, Nathalie, JOLY, Thierry, LYNCH, Cian, MARCY, Guillaume, MASFARAUD, Etienne, MAYÈRE, Chloé, MOULIN, Anaïs, RAINETEAU, Olivier, ROGNARD, Cloé, SAVATIER, Pierre, SERRANO MARUGÁN, Manuel, WIANNY, Florence. Apoptosis, G1 Phase Stall, and Premature Differentiation Account for Low Chimeric Competence of Human and Rhesus Monkey Naive Pluripotent Stem Cells. _Stem Cell Reports_. 2021. Vol. 16, núm. 1, pàgs. 56-74. [consulta: 25 de febrer de 2026]. [Disponible a: https://hdl.handle.net/2445/174577]

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