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2014-04-09

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cc-by (c) Cantó, Elisabet et al., 2014
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/55853

Interleukin-19 impairment in active Crohn's disease patients

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http://dx.doi.org/10.1371/journal.pone.0093910

Abstract

The exact function of interleukin-19 (IL-19) on immune response is poorly understood. In mice, IL-19 up-regulates TNFalpha and IL-6 expression and its deficiency increases susceptibility to DSS-induced colitis. In humans, IL-19 favors a Th2 response and is elevated in several diseases. We here investigate the expression and effects of IL-19 on cells from active Crohn"s disease (CD) patient. Twenty-three active CD patients and 20 healthy controls (HC) were included. mRNA and protein IL-19 levels were analyzed in monocytes. IL-19 effects were determined in vitro on the T cell phenotype and in the production of cytokines by immune cells. We observed that unstimulated and TLR-activated monocytes expressed significantly lower IL-19 mRNA in active CD patients than in HC (logFC =21.97 unstimulated; 21.88 with Pam3CSK4; and 21.91 with FSL-1; p<0.001). These results were confirmed at protein level. Exogenous IL-19 had an anti-inflammatory effect on HC but not on CD patients. IL-19 decreased TNFalpha production in PBMC (850.7675.29 pg/ml vs 2626.06350 pg/ml; p<0.01) and increased CTLA4 expression (22.0461.55% vs 13.9862.05%; p<0.05) and IL-4 production (32.568.9 pg/ml vs 13.562.9 pg/ml; p<0.05) in T cells from HC. IL-10 regulated IL-19 production in both active CD patients and HC. We observed that three of the miRNAs that can modulate IL-19 mRNA expression, were up-regulated in monocytes from active CD patients. These results suggested that IL-19 had an anti-inflammatory role in this study. Defects in IL-19 expression and the lack of response to this cytokine could contribute to inflammatory mechanisms in active CD patients.

Subject

Cèl·lules T, Citometria de fluxe, Citoquines, Leucòcits, Malaltia de Crohn, Micro RNAs, Resposta immunitària, RNA

Subject (English)

T cells, Flow cytometry, Cytokines, Leucocytes, Crohn's disease, MicroRNAs, Immune response, RNA

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citation

CANTÓ, Elisabet, et al. Interleukin-19 impairment in active Crohn's disease patients. PLoS One. 2014. Vol. 9, num. 4, pags. e93910. ISSN 1932-6203. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/55853

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