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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177048
Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
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BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA.
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SOLÉ FERRÉ, Anna, MENCÍA TRINCHANT, Núria, VILLALOBOS ALBERÚ, Xenia, NOÉ MATA, Verónica, CIUDAD I GÓMEZ, Carlos julián. Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays. _BMC Research Notes_. 2013. Vol. 6, núm. 1, pàgs. 454. [consulta: 20 de gener de 2026]. ISSN: 1756-0500. [Disponible a: https://hdl.handle.net/2445/177048]