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2013-10-12

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cc-by (c) Solé Ferré, Anna et al., 2013
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177048

Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays

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Recurs relacionat

https://doi.org/10.1186/1756-0500-6-454

Resum

BACKGROUND: MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3[prime]-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3[prime]-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA. FINDINGS: RNA molecules corresponding to miR-224 and to the 3[prime]-UTR of SLC4A4 were incubated together and their interaction studied under different binding conditions using electrophoretic mobility shift assays. A direct and specific interaction between miR-224 and SLC4A4 mRNA was observed. This interaction was abolished in the presence of competitors. CONCLUSIONS: In this study, we explored a new application for the electrophoretic mobility shift assay and we demonstrated that it is a useful alternative method to assess, in a direct and specific manner, whether a miRNA binds to a specific predicted target mRNA.

Matèries

Micro RNAs, RNA, Dianes farmacològiques, Expressió gènica

Matèries (anglès)

MicroRNAs, RNA, Drug targeting, Gene expression

Citació

Col·leccions

Articles publicats en revistes (Bioquímica i Fisiologia)
Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

SOLÉ FERRÉ, Anna, et al. Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays. BMC Research Notes. 2013. Vol. 6, num. 1, pags. 454. ISSN 1756-0500. [consulted: 14 of August of 2026]. Available at: https://hdl.handle.net/2445/177048

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