Files
Document type
ArticleVersion
Published versionPublication date
All rights reserved
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/25804
Thermal activation and ATP dependence of the cytoskeleton remodeling dynamics
Journal Title
Director/Tutor
Additional contribution
Journal ISSN
Volume Title
Abstract
The cytoskeleton (CSK) is a nonequilibrium polymer network that uses hydrolyzable sources of free energy such as adenosine triphosphate (ATP) to remodel its internal structure. As in inert nonequilibrium soft materials, CSK remodeling has been associated with structural rearrangements driven by energy-activated processes. We carry out particle tracking and traction microscopy measurements of alveolar epithelial cells at various temperatures and ATP concentrations. We provide the first experimental evidence that the remodeling dynamics of the CSK is driven by structural rearrangements over free-energy barriers induced by thermally activated forces mediated by ATP. The measured activation energy of these forces is ~40kBTr (kB being the Boltzmann constant and Tr being the room temperature). Our experiments provide clues to understand the analogy between the dynamics of the living CSK and that of inert nonequilibrium soft materials.
Related resource
Subject
Subject (English)
Citation
Citation
FARRÉ VENTURA, Ramon and NAVAJAS NAVARRO, Daniel. Thermal activation and ATP dependence of the cytoskeleton remodeling dynamics. Physical Review E. 2009. Vol. 79, num. num 5, pags. 51920/1-51920/7. ISSN 1063-651X. [consulted: 9 of October of 2026]. Available at: https://hdl.handle.net/2445/25804