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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/199748
Green extraction of antioxidant compounds from olive tree leaves based on naturaldeep eutectic solvents
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Agri-food industries generate a large amount of waste that offers great revalorization opportunities within the circular economy framework. In recent years, new methodologies for the extraction of compounds with more eco-friendly solvents have been developed, such as the case of natural deep eutectic solvents (NADES). In this study, a methodology for extracting phenolic compounds from olive tree leaves using NADES has been optimized. The conditions established as the optimal rely on a solvent composed of choline chloride and glycerol at a molar ratio of 1:5 with 30% water. The extraction was carried out at 80 °C for 2 h with constant agitation. The extracts obtained have been analyzed by high-performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) in MRM mode. The comparison with conventional ethanol/water extraction has shown that NADES, a more environmentally friendly alternative, has improved extraction efficiency. The main polyphenols identified in the NADES extract were Luteolin-7-O-glucoside, Oleuropein, 3-Hydroxytyrosol, Rutin, and Luteolin at the concentrations of 262, 173, 129, 34, and 29 mg kg−1 fresh weight, respectively.
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MIR-CERDÀ, Aina, et al. Green extraction of antioxidant compounds from olive tree leaves based on naturaldeep eutectic solvents. Antioxidants. 2023. Vol. 12, num. 5, pags. 995. ISSN 2076-3921. [consulted: 12 of June of 2026]. Available at: https://hdl.handle.net/2445/199748