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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/177601
Comprehensive high performance thin layer chromatography (HPTLC) fingerprinting in quality control of herbal drugs, preparations and products
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[eng] Quality control of herbals has its roots in the study of morphoanatomic and organoleptic characters. Nevertheless, in the last century, with the evolution of analytical chemistry, the quality control rapidly evolved from elementary tests to the use of sophisticated instruments combined with software for data management. In the current days, many authorities and organizations recommend a suite of tests, featuring many of these instruments, to evaluate quality of herbal products. HPTLC offers a comprehensive set of data that can be used not only for identification but also to evaluate the purity and content of herbal drugs, herbal preparations, and herbal products. The objective of this doctoral thesis was to explore in-depth the capacities of HPTLC and develop applications for quality control of herbals, far beyond simple identification of the herbal drugs, preparations, and products. For that, five studies were developed. In the first study, the quality of herbal drugs, preparations, and products from milk thistle fruit, coneflower root and aerial parts and black cohosh root, regulated under food supplements or medicines were evaluated with existing HPTLC methods. The suitability of these methods, using the entire fingerprint and several detection modes, as a tool for detecting quality problems, mainly adulterations, was confirmed. In the second study, the comprehensive HPTLC fingerprinting concept was developed with the goal of simplifying the quality control process. This concept combines the qualitative and quantitative information of HPTLC images, obtained in a single analysis, to evaluate the identity, purity and content of herbals. The possibilities of applying it to identify an herbal drug, detect mixtures with re¬lated species (purity), and develop a minimum content test of an analytical marker were demonstrated in Angelica gigas root. In the third study, the application of comprehensive HPTLC fingerprinting aimed to go one step beyond in the test for adulterants and to evaluate the use of the HPTLC for purity limit tests. This approach was evaluated with sam¬ples of ginkgo leaf and extracts, commercialized as food supplements in different countries. This study demonstrated that the information contained in the HPTLC finger¬prints was suitable for verifying levels of rutin and quercetin, providing results similar to that of the HPLC limit test. It was also useful for detecting mixtures of ginkgo products not only with rutin and quercetin but also with buckwheat herb and sophora (flower bud or fruit). In the fourth study, it was evaluated the use of comprehensive HPTLC fingerprinting as an alternative method to the current HPLC assay of markers of TCM drugs in the Ph. Eur. The goal of this project was to simplify the determination of content and thus reducing the number of tests to be performed during quality control. For this evaluation, two TCM herbal drugs were chosen by the experts of the TCM working party of the Ph. Eur.: Fritillaria thunbergii bulbs and corydalis rhizome. In both cases, comprehensive HPTLC fingerprinting was proven useful for identification and minimum content testing in one single analysis. The fifth study goes one step beyond in the content determination. While the previous studies focused in the quantification of single markers, this study aimed to apply comprehensive HPTLC fingerprinting to quantify a group of constituents in an herbal drug, as an example of a more holistic assessment of quality. This determination was combined with the tests for purity and identity. To illustrate this concept, Ganoderma lucidum fruiting body was chosen. In this work, HPTLC proved to be a useful technique for routine quality control of herbal drugs, preparations and products. As demonstrated, it can simplify this process by applying the concept of comprehensive HPTLC fingerprinting. A detailed guideline of how to develop, validate and apply comprehensive HPTLC fingerprinting methods for routine quality control of herbals has been elaborated and is also included in the thesis.
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ARRUDA FROMMENWILER, Débora. Comprehensive high performance thin layer chromatography (HPTLC) fingerprinting in quality control of herbal drugs, preparations and products. [consulta: 12 de desembre de 2025]. [Disponible a: https://hdl.handle.net/2445/177601]