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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/175880
Computational study of the active center of an endo-B-1,4-mannosidase, a carbohydrate-degrading enzyme
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Glycoside hydrolases, also named glycosidases (GHs), are enzymes responsible for processing carbohydrates by hydrolyzing their glycosidic bond linkages. These enzymes operate either by retention or inversion of the configuration of the anomeric carbon. Generally speaking, two carboxylate-based protein residues, which perform the functions of acid/base and nucleophilic catalysts, are directly involved in the reaction. In this project, the pKa of the catalytic residues will be calculated by means of different computational methods and the active site of a bacterial endo-B-1,4-mannanase of GH family 134 will be studied also by computer simulation to understand how the protonation state of these two carboxylate residues can impact the structure and dynamics of the enzyme. In addition, the configuration of the cleaved sugar ring during the simulation will be mapped
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Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2020, Tutora: Carme Rovira Virgili
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SILVESTRE BARCELÓ, Ricard. Computational study of the active center of an endo-B-1,4-mannosidase, a carbohydrate-degrading enzyme. [consulted: 12 of August of 2026]. Available at: https://hdl.handle.net/2445/175880