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cc-by-nc-nd (c) Pages-Rebull, Josep, et al., 2025
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/225641

Pulsed amperometric detection: Extending the applicability of HPLC to substances with two-step redox processes.

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Electrochemical detection coupled to liquid chromatography provides high selectivity compared to optical detection due to the lower number of electrochemically active compounds. Nevertheless, not all electrochemically active substances may be detected with the classical amperometric detection that involves the application of a single potential. For instance, in flow conditions, the main biomarkers of cinnamon (eugenol, coumarin, cinnamaldehyde and cinnamic acid) require an oxidation step prior to their amperometric detection. Pulsed amperometric detection (PAD) is proposed to address the electrochemical determination of substances with such complex redox behaviour in flow conditions because it allows the application of multiple potential steps that mimic the sequential stages that would be performed in static voltammetric measurements. PAD waveform has been optimized resulting in two scenarios (tox>> tred and tox <

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PAGES-REBULL, Josep, PÉREZ RÀFOLS, Clara, SERRANO I PLANA, Núria, DÍAZ CRUZ, José manuel. Pulsed amperometric detection: Extending the applicability of HPLC to substances with two-step redox processes.. _Microchemical Journal_. 2025. Vol. 219, núm. 116086. [consulta: 3 de febrer de 2026]. ISSN: 0026-265X. [Disponible a: https://hdl.handle.net/2445/225641]

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