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2014-06

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/54104

Lysine-based surfactants in nanovesicle formulations: the role of cationic charge position and hydrophobicity in in vitro cytotoxicity and intracellular delivery

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Recurs relacionat

http://dx.doi.org/10.3109/17435390.2013.793779

Resum

Understanding nanomaterial interactions within cells is of increasing importance for assessing their toxicity and cellular transport. Here, we developed nanovesicles containing bioactive cationic lysine-based amphiphiles, and assessed whether these cationic compounds increase the likelihood of intracellular delivery and modulate toxicity. We found different cytotoxic responses among the formulations, depending on surfactant, cell line and endpoint assayed. The induction of mitochondrial dysfunction, oxidative stress and apoptosis were the general mechanisms underlying cytotoxicity. Fluorescence microscopy analysis demonstrated that nanovesicles were internalized by HeLa cells, and evidenced that their ability to release endocytosed materials into cell cytoplasm depends on the structural parameters of amphiphiles. The cationic charge position and hydrophobicity of surfactants determine the nanovesicle interactions within the cell and, thus, the resulting toxicity and intracellular behavior after cell uptake of the nanomaterial. The insights into some toxicity mechanisms of these new nanomaterials contribute to reducing the uncertainty surrounding their potential health hazards.

Matèries

Toxicologia, Materials nanoestructurats, Sistemes d'alliberament de medicaments, Cultiu cel·lular, Citotoxicitat per mediació cel·lular

Matèries (anglès)

Toxicology, Nanostructured materials, Drug delivery systems, Cell culture, Cell-mediated cytotoxicity

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Articles publicats en revistes (Bioquímica i Fisiologia)

Citació

NOGUEIRA, Daniele R., et al. Lysine-based surfactants in nanovesicle formulations: the role of cationic charge position and hydrophobicity in in vitro cytotoxicity and intracellular delivery. Nanotoxicology. 2013. Vol. 8, num. 4, pags. 404-421. ISSN 1743-5390. [consulted: 2 of June of 2026]. Available at: https://hdl.handle.net/2445/54104

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