Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

BARRANTES CEPAS MAR_4253552_assignsubmission_file_TFG-Barrantes-Cepas-Mar.pdf (2.29 MB)

Document type

Bachelor thesis

Publication date

2021-07

Publication license

cc-by-nc-nd (c) Barrantes, 2021
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/180597

Differences in activity and functional connectivity between bi-dimensional and three-dimensional neuronal cultures

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

Abstract

Three-dimensional (3D) neuronal cultures can provide a more desirable in vitro ap-proach to emulate brain-like conditions. To prove it, 2D and 3D neuronal cultures were compared. During different days in vitro (DIV) after formation, spontaneous neuronal activity was recorded through calcium fluorescence imaging for data extraction. We show that activity in 3D cultures is higher and more varied. These cultures are functionally organized in modules that are spatially localized. In comparison, 2D cultures show less variability in activity, higher connectivity between neurons and a poor organization in modules. We ascribe the differences to the capacity of 3D cul-tures to form connections of short distance, favouring small microcircuits that coexist, while the 2D cultures have long–range connections that shape a strongly coupled and rigid system

Description

Treballs Finals de Grau de Física, Facultat de Física, Universitat de Barcelona, Curs: 2021, Tutor: Jordi Soriano Fradera

Subject

Cultiu cel·lular, Xarxes neuronals (Neurobiologia), Treballs de fi de grau

Subject (English)

Cell culture, Neural networks (Neurobiology), Bachelor's theses

Citation

Collections

Treballs Finals de Grau (TFG) - Física

Citation

BARRANTES CEPAS, Mar. Differences in activity and functional connectivity between bi-dimensional and three-dimensional neuronal cultures. [consulted: 18 of August of 2026]. Available at: https://hdl.handle.net/2445/180597

Export metadata

JSON - METS

Share record