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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/112377
A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea
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Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time similar to 6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1 alpha (HIF-1 alpha) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch.
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CAMPILLO, Noelia, JORBA, Ignasi, SCHAEDEL, Laura, CASALS MONTSERRAT, Blai, GOZAL, David, FARRÉ VENTURA, Ramon, ALMENDROS LÓPEZ, Isaac, NAVAJAS NAVARRO, Daniel. A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea. _Frontiers in Physiology_. 2016. Vol. 7, núm. 319. [consulta: 8 de gener de 2026]. ISSN: 1664-042X. [Disponible a: https://hdl.handle.net/2445/112377]