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2016-07-29

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cc-by (c) Campillo, Noelia et al., 2016
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/112377

A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea

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Recurs relacionat

https://doi.org/10.3339/fphys.2016.00319

Resum

Intermittent hypoxia (IH), a hallmark of obstructive sleep apnea (OSA), plays a critical role in the pathogenesis of OSA-associated morbidities, especially in the cardiovascular and respiratory systems. Oxidative stress and inflammation induced by IH are suggested as main contributors of end-organ dysfunction in OSA patients and animal models. Since the molecular mechanisms underlying these in vivo pathological responses remain poorly understood, implementation of experimental in vitro cell-based systems capable of inducing high-frequency IH would be highly desirable. Here, we describe the design, fabrication, and validation of a versatile chip for subjecting cultured cells to fast changes in gas partial pressure and to cyclic stretch. The chip is fabricated with polydimethylsiloxane (PDMS) and consists of a cylindrical well-covered by a thin membrane. Cells cultured on top of the membrane can be subjected to fast changes in oxygen concentration (equilibrium time similar to 6 s). Moreover, cells can be subjected to cyclic stretch at cardiac or respiratory frequencies independently or simultaneously. Rat bone marrow-derived mesenchymal stem cells (MSCs) exposed to IH mimicking OSA and cyclic stretch at cardiac frequencies revealed that hypoxia-inducible factor 1 alpha (HIF-1 alpha) expression was increased in response to both stimuli. Thus, the chip provides a versatile tool for the study of cellular responses to cyclical hypoxia and stretch.

Matèries

Síndromes d'apnea del son, Oxigen en l'organisme, Respiració

Matèries (anglès)

Sleep apnea syndromes, Oxygen in the body, Respiration

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Articles publicats en revistes (Biomedicina)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)
Articles publicats en revistes (Institut de Bioenginyeria de Catalunya (IBEC))

Citació

CAMPILLO, Noelia, et al. A Novel chip for cyclic stretch and intermittent hypoxia cell exposures mimicking obstructive sleep apnea. Frontiers in Physiology. 2016. Vol. 7, num. 319. ISSN 1664-042X. [consulted: 27 of May of 2026]. Available at: https://hdl.handle.net/2445/112377

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