Carregant...
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/185457
Towards Cellular Ultrastructural Characterization in Organ-on-a-Chip by Transmission Electron Microscopy
Títol de la revista
ISSN de la revista
Títol del volum
Resum
Organ-on-a-chip technology is a 3D cell culture breakthrough of the last decade. This rapidly developing field of bioengineering intertwined with microfluidics provides new insights into disease development and preclinical drug screening. So far, optical and fluorescence microscopy are the most widely used methods to monitor and extract information from these models. Meanwhile transmission electron microscopy (TEM), despite its wide use for the characterization of nanomaterials and biological samples, remains unexplored in this area. In our work we propose a TEM sample preparation method, that allows to process a microfluidic chip without its prior deconstruction, into TEM-compatible specimens. We demonstrated preparation of tumor blood vessel-on-a-chip model and consecutive steps to preserve the endothelial cells lining microfluidic channel, for the chip’s further transformation into ultrathin sections. This approach allowed us to obtain cross-sections of the microchannel with cells cultured inside, and to observe cell adaptation to the channel geometry, as well as the characteristic for endothelial cells tight junctions. The proposed sample preparation method facilitates the electron microscopy ultrastructural characterization of biological samples cultured in organ-on-a-chip device.
Descripció
Matèries (anglès)
Citació
Citació
GLINKOWSKA MARES, Adrianna, FEINER GRACIA, Natalia, MUELA, Yolanda, MARTÍNEZ, Gema, DELGADO, Lídia, ALBERTAZZI, Lorenzo, PUJALS RIATÓS, Silvia. Towards Cellular Ultrastructural Characterization in Organ-on-a-Chip by Transmission Electron Microscopy. _Applied Nano_. 2021. Vol. 2, núm. 4, pàgs. 289-302. [consulta: 26 de novembre de 2025]. ISSN: 2673-3501. [Disponible a: https://hdl.handle.net/2445/185457]