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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/214714
Gene regulation in activated microglia by adenosine A3 receptor agonists: a transcriptomics study
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Most neurodegenerative disorders, including the two most common, Alzheimer’s disease (AD) and Parkinson’s disease (AD), course with activation of microglia, the resident innate immune cells of the central nervous system. A3 adenosine receptor (A3R) agonists have been proposed to be neuroprotective by regulating the phenotype of activated microglia. RNAseq was performed using samples isolated from lipopolysaccharide/interferon-γ activated microglia treated with 2-Cl-IB-MECA, a selective A3R agonist. The results showed that the number of negatively regulated genes in the presence of 2-Cl-IB-MECA was greater than the number of positively regulated genes. Gene ontology enrichment analysis showed regulation of genes participating in several cell processes, including those involved in immune-related events. Analysis of known and predicted protein-protein interactions showed that Smad3 and Sp1 are transcription factors whose genes are regulated by A3R activation. Under the conditions of cell activation and agonist treatment regimen, 2-Cl-IB-MECA did not lead to any tendency to favor the expression of genes related to neuroprotective microglia (M2). Supplementary Information The online version contains supplementary material available at 10.1007/s11302-022-09916-9. Keywords: Alzheimer’s disease, Parkinson’s disease, Neurodegeneration, Adenosine, Receptors, Microglia, Neuroinflammation
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LILLO, Alejandro, SERRANO-MARÍN, Joan, LILLO, Jaume, RAÏCH, Iu, NAVARRO BRUGAL, Gemma, FRANCO FERNÁNDEZ, Rafael. Gene regulation in activated microglia by adenosine A3 receptor agonists: a transcriptomics study. _Purinergic Signalling_. 2023. [consulta: 22 de gener de 2026]. ISSN: 1573-9538. [Disponible a: https://hdl.handle.net/2445/214714]