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Study of light-induced formation of photodimers in the i-motif nucleic acid structure by rapid-scan FTIR difference spectroscopy and hybrid hard- and soft-modelling
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The i-motif is a DNA structure formed by cytosine-rich sequences, very relevant from a biochemical point of view and potentially useful in Nanotechnology as pH-sensitive nanodevices or nanomotors. To provide a different view on the structural changes and dynamics of direct excitation processes involving i-motif structures, the use of rapid scan FTIR spectroscopy is proposed. Hybrid hard- and soft-modelling based on the Multivariate Curve Resolution by Alternating least squares (MCR-ALS) algorithm has been used for the resolution of rapid-scan FTIR spectra and the interpretation of the photochemically induced time-dependent conformational changes of i-motif structures. The hybrid hard- and soft-modelling version of MCR-ALS (HS-MCR), which allows the introduction of kinetic models to describe the process behavior, provides also rate constants associated with the transitions modeled. The results show that i-motif structures formed by short DNA sequences present higher structural changes upon UV irradiation than those formed by long sequences with additional structural stabilizing elements, such as hairpins.
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BENABOU ZDAOU, Sanae, RUCKEBUSCH, Cyril, SLIWA, Michel, AVIÑÓ ANDRÉS, Anna, ERITJA I CASADELLÀ, Ramon, GARGALLO GÓMEZ, Raimundo, JUAN CAPDEVILA, Anna de. Study of light-induced formation of photodimers in the i-motif nucleic acid structure by rapid-scan FTIR difference spectroscopy and hybrid hard- and soft-modelling. _Physical Chemistry Chemical Physics_. 2018. Vol. 20, núm. 19635-19646. [consulta: 14 de febrer de 2026]. ISSN: 1463-9076. [Disponible a: https://hdl.handle.net/2445/127350]