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2021-01-19

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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/204223

Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions

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https://doi.org/10.1093/jac/dkaa459

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Objectives: To characterize the clonal spread of carbapenem-resistant Klebsiella pneumoniae and Escherichia coli isolates between different healthcare institutions in Catalonia, Spain. Methods: Antimicrobial susceptibility was tested by disc diffusion. MICs were determined by gradient diffusion or broth microdilution. Carbapenemase production was confirmed by lateral flow. PCR and Sanger sequencing were used to identify the allelic variants of resistance genes. Clonality studies were performed by PFGE and MLST. Plasmid typing, conjugation assays, S1-PFGE plus Southern blotting and MinION Oxford Nanopore sequencing were used to characterize resistance plasmids. Results: Twenty-nine carbapenem-resistant isolates recovered from three healthcare institutions between January and November 2016 were included: 14 K. pneumoniae isolates from a tertiary hospital in the south of Catalonia (hospital A); 2 K. pneumoniae isolates from a nearby healthcare centre; and 12 K. pneumoniae isolates and 1 E. coli isolate from a tertiary hospital in Barcelona (hospital B). The majority of isolates were resistant to all antimicrobial agents, except colistin, and all were NDM producers. PFGE identified a major K. pneumoniae clone (n = 27) belonging to ST147 and co-producing NDM-1 and CTX-M-15, with a few isolates also harbouring blaOXA-48. Two sporadic isolates of K. pneumoniae ST307 and E. coli ST167 producing NDM-7 were also identified. blaNDM-1 was carried in two related IncR plasmid populations and blaNDM-7 in a conjugative 50 kb IncX3 plasmid. Conclusions: We report the inter-hospital dissemination of XDR high-risk clones of K. pneumoniae and E. coli associated with the carriage of small, transferable plasmids harbouring blaNDM genes.

Matèries

Escheríchia coli, Medicaments antibacterians, Microbiologia mèdica, Klebsiella pneumoniae

Matèries (anglès)

Escherichia coli, Antibacterial agents, Medical microbiology, Klebsiella pneumoniae

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Articles publicats en revistes (ISGlobal)
Articles publicats en revistes (Fonaments Clínics)

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MARÍ ALMIRALL, Marta, COSGAYA CASTRO, Clara, PITART, Cristina, VIÑES, Joaquim, MUÑOZ, Laura, CAMPO, Irene, CUSCÓ, Anna, RODRÍGUEZ SERNA, Laura, SANTANA, Gemina, RÍO, Ana del, FRANCINO, Olga, CIRUELA, Pilar, PUJOL, Isabel, BALLESTER, Frederic, MARCO, Francesc, MARTÍNEZ, José antonio (martínez martínez), SORIANO, Álex, VILA ESTAPÉ, Jordi, ROCA, Ignasi, MERCyCAT Study Group. Dissemination of NDM-producing Klebsiella pneumoniae and Escherichia coli high-risk clones in Catalan healthcare institutions. _Journal of Antimicrobial Chemotherapy_. 2021. Vol. 76, núm. 2, pàgs. 345-354. [consulta: 21 de gener de 2026]. ISSN: 0305-7453. [Disponible a: https://hdl.handle.net/2445/204223]

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