Miniatura

Fitxers

678566.pdf (1.24 MB)

Tipus de document

Article

Versió

Versió acceptada

Data de publicació

2018-02-27

Tots els drets reservats

Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/157802

Hierarchical structures of anodised cold gas sprayed titanium coatings

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1080/00202967.2018.1419625

Resum

Three different surface treatments on a Ti6Al4V alloy have been in vitro tested for possible application in cementless joint prosthesis; all of them involve the novelty of using the Cold Spray technology for their deposition: (i) an as-sprayed highly rough titanium and, followed by the deposition of a thin hydroxyapatite layer with (ii) microcrystalline or (iii) nanocrystalline structure. Primary human osteoblasts extracted from knee were seeded onto surfaces and cell viability using MTS and LIFE/DEAD assays, osteoblasts differentiation by alkaline phosphatase (ALP) quantification as well as cell morphology were tested at 1, 7 and 14 days of cell culture. Different cell morphologies between titanium and hydroxyapatite surfaces were exhibited; at 1 day of cell culture, cells on the titanium coating were spread and flattened, expanding the filopodia actin filaments in all directions, while cells on the hydroxyapatite coatings showed round like-shape morphology due to a slower attachment. Higher cell viability was detected at all times of cell culture on titanium coating due to a better attachment at 1 day. However, from 7 days of cell culture, cells on hydroxyapatite showed good attachment onto surfaces and highly increased their proliferation, mostly on nanocrystalline, achieving similar cell viability levels than titanium coatings. ALP levels were significantly higher in titanium, in part, because of greatest cell number. Overall, the best cell functional results were obtained on titanium coatings whereas microcrystalline hydroxyapatite presented the worst cellular parameters. However, results indicate that nanocrystalline hydroxyapatite coatings may achieve promising results for the faster cell proliferation once cells are attached on the surface.

Matèries

Titani, Revestiments, Nanotubs, Materials biomèdics

Matèries (anglès)

Titanium, Coatings, Nanotubes, Biomedical materials

Citació

Col·leccions

Articles publicats en revistes (Ciència dels Materials i Química Física)

Citació

MARTÍN VILARDELL, Anna, CINCA I LUIS, Núria, PACHECO, I., SANTIVERI CARRERA, Carlos, DOSTA PARRAS, Sergi, GUILEMANY, J. m. (josé maría), SARRET I PONS, Maria, MULLER JEVENOIS, Carlos. Hierarchical structures of anodised cold gas sprayed titanium coatings. _Transactions of the Institute of Metal Finishing_. 2018. Vol. 96, núm. 2, pàgs. 71-78. [consulta: 21 de gener de 2026]. ISSN: 0020-2967. [Disponible a: https://hdl.handle.net/2445/157802]

Exportar metadades

JSON - METS

Compartir registre