Amb motiu del tancament d'estiu, la validació de documents es reprendrà a partir del 28 d'agost de 2026. Disculpeu les molèsties.
Con motivo del cierre de verano, la validación de documentos se reanudará a partir del 28 de agosto de 2026. Disculpad las molestias
Due to the summer closure, document validation will resume starting August 28, 2026. We apologize for any inconvenience.

Files

TFG_QU Cortés Valle, Sílvia.pdf (796.88 KB)

Document type

Bachelor thesis

Publication date

2020-07

Publication license

cc-by-nc-nd (c) Cortés, 2020
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/175993

Characterization of HILIC systems: underivatized silica as stationary phase

Journal Title

Authors

Director/Tutor

Journal ISSN

Volume Title

Related resource

Abstract

Hydrophilic interaction liquid chromatography (HILIC) is a type of chromatography especially used for the analysis of polar substances, using mobile phases with a high content of organic solvent and polar stationary phases. Nowadays, its retention mechanism still under study due to its complexity. In this work, the Abraham’s model based on linear free energy relationships (LFER) has been used in order to characterize the study column (Kinetex® with silica support), obtaining information about the mobile phase volume inside the column and the retention mode under certain working conditions. As a way of characterizing this column, the behavior of different homologous series (n-alkyl benzenes, n-alkyl phenones and n-alkyl ketones) has been analyzed with different proportions of mobile phase (acetonitrile and water mixture). According to the mobile phase composition, significative variations on the retention mode and on the mobile phase volume inside the column can be observed. In fact, this study could be useful for the determination of possible hold-up volume markers, depending on the prevailing retention mode

Description

Treballs Finals de Grau de Química, Facultat de Química, Universitat de Barcelona, Any: 2020, Tutors: Martí Rosés Pascual, Xavier Subirats Vila

Subject

Cromatografia líquida d’interacció hidrofílica, Sèrie homòloga, Treballs de fi de grau

Subject (English)

Hydrophilic interaction liquid chromatography, Homologous series, Bachelor's theses

Citation

Collections

Treballs Finals de Grau (TFG) - Química

Citation

CORTÉS VALLE, Sílvia. Characterization of HILIC systems: underivatized silica as stationary phase. [consulted: 11 of August of 2026]. Available at: https://hdl.handle.net/2445/175993

Export metadata

JSON - METS

Share record