Files
Document type
ArticleVersion
Published versionPublication date
Publication license
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/139271
TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4
Journal Title
Director/Tutor
Journal ISSN
Volume Title
Related resource
Abstract
Mechanically induced signaling pathways are important drivers of tumor progression. However, if and how mechanical signals affect metastasis or therapy response remains poorly understood. We previously found that the channel-kinase TRPM7, a regulator of cellular tension implicated in mechano-sensory processes, is required for breast cancer metastasis in vitro and in vivo. Here, we show that TRPM7 contributes to maintaining a mesenchymal phenotype in breast cancer cells by tensional regulation of the EMT transcription factor SOX4. The functional consequences of SOX4 knockdown closely mirror those produced by TRPM7 knockdown. By traction force measurements, we demonstrate that TRPM7 reduces cytoskeletal tension through inhibition of myosin II activity. Moreover, we show that SOX4 expression and downstream mesenchymal markers are inversely regulated by cytoskeletal tension and matrix rigidity. Overall, our results identify SOX4 as a transcription factor that is uniquely sensitive to cellular tension and indicate that TRPM7 may contribute to breast cancer progression by tensional regulation of SOX4.
Subject
Subject (English)
Citation
Citation
KUIPERS, Arthur J., et al. TRPM7 controls mesenchymal features of breast cancer cells by tensional regulation of SOX4. Biochimica et Biophysica Acta. 2018. Vol. 1864, num. 7, pags. 2409-2419. ISSN 0006-3002. [consulted: 9 of August of 2026]. Available at: https://hdl.handle.net/2445/139271