Fitxers

864663.pdf (1.49 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2024

Llicència de publicació

cc-by-nc-nd (c) Elsevier, 2024
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/229093

Rapid detection and quantification of milk adulteration using MALDI-MSprotein profiling and multivariate calibration

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1016/j.jfca.2024.106147

Resum

The adulteration of high-value milks with low-value milks is a common fraudulent practice in the dairy industry. This not only results in economic or quality prejudice, but also poses a potential threat to individuals with sensitivities or allergies. Profiling the major whey and casein milk proteins can be a crucial tool in combating this malpractice, given that each mammal species exhibits a unique protein fingerprint. In this study, we employed matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-MS) as a rapid and straightforward method for profiling proteins from bovine, caprine, and ovine milks. Subsequently, we analyzed binary mixtures of goat and sheep milks with bovine milk to establish multivariate calibration models using partial least-squares (PLS) to quantify the adulteration of goat and sheep milks. Proteins were identified according to their molecular mass (Mr) and their intensities were considered as multivariate data. To achieve satisfactory calibration and validation results, normalization through variable augmentation was necessary, enabling the quantification of bovine milk across the entire adulteration range. This uncomplicated approach based on MALDI-MS protein profiling and PLS shows significant potential for qualitative and quantitative assessments of milk adulteration.

Matèries

Proteïnes de la llet, Espectrometria de masses, Llet

Matèries (anglès)

Milk proteins, Mass spectrometry, Milk

Citació

Col·leccions

Articles publicats en revistes (Enginyeria Química i Química Analítica)
Articles publicats en revistes (Institut de Recerca en Nutrició i Seguretat Alimentària (INSA·UB))

Citació

TEHRANI, Tahereh, PONT VILLANUEVA, Laura and BENAVENTE MORENO, Fernando J. (Julián). Rapid detection and quantification of milk adulteration using MALDI-MSprotein profiling and multivariate calibration. Journal Of Food Composition And Analysis. 2024. Vol. 130, num. 106147. ISSN 0889-1575. [consulted: 22 of May of 2026]. Available at: https://hdl.handle.net/2445/229093

Exportar metadades

JSON - METS

Compartir registre