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Accepted version

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2024-09

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cc-by-nc-nd (c) Elsevier B.V., 2024
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/218744

Decay of RNA and infectious SARS-CoV-2 and murine hepatitis virus in wastewater

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https://doi.org/10.1016/j.scitotenv.2024.173877

Abstract

Wastewater-based epidemiology (WBE) has been an important tool for population surveillance during the COVID-19 pandemic and continues to play a key role in monitoring SARS-CoV-2 infection levels following reductions in national clinical testing schemes. Studies measuring decay profiles of SARS-CoV-2 in wastewater have underscored the value of WBE, however investigations have been hampered by high biosafety requirements for SARS-CoV-2 infection studies. Therefore, surrogate viruses with lower biosafety standards have been used for SARS-CoV-2 decay studies, such as murine hepatitis virus (MHV), but few studies have directly compared decay rates of both viruses. We compared the persistence of SARS-CoV-2 and MHV in wastewater, using 50 % tissue culture infectious dose (TCID50) and reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays to assess infectious virus titre and viral gene markers, respectively. Infectious SARS-CoV-2 and MHV indicate similar endpoints, however observed early decay characteristics differed, with infectious SARS-CoV-2 decaying more rapidly than MHV. We find that MHV is an appropriate infectious virus surrogate for viable SARS-CoV-2, however inconsistencies exist in viral RNA decay parameters, indicating MHV may not be a suitable nucleic acid surrogate across certain temperature regimes. This study highlights the importance of sample preparation and the potential for decay rate overestimation in wastewater surveillance for SARS-CoV-2 and other pathogens.

Subject

Aigües residuals, SARS-CoV-2, Seguiment ambiental

Subject (English)

Sewage, SARS-CoV-2, Environmental monitoring

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Articles publicats en revistes (Genètica, Microbiologia i Estadística)

Citation

PURVES, Kevin, et al. Decay of RNA and infectious SARS-CoV-2 and murine hepatitis virus in wastewater. Science of the Total Environment. 2024. Vol. 944, num. 1-9. ISSN 0048-9697. [consulted: 19 of August of 2026]. Available at: https://hdl.handle.net/2445/218744

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