Miniatura

Fitxers

633810.pdf (1.54 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2014-02-06

Llicència de publicació

cc-by-nc-sa (c) Ventayol Espinazo, Marina et al., 2014
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/133452

miRNA let-7e targeting MMP9 is involved in adipose-derived stem cell differentiation toward epithelia

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1038/cddis.2014.2

Resum

miRNA let-7e is involved in stem cell differentiation, and metalloproteinases are among its potential target genes. We hypothesized that the inhibitory action of let-7e on regulation of MMP9 expression could represent a crucial mechanism during differentiation of adipose-derived stem cells (ASCs). ASCs were differentiated with all-trans retinoic acid (ATRA) to promote differentiation, and the effect of let-7 silencing during differentiation was tested. Results indicate that ASCs cultured with ATRA differentiated into cells of the epithelial lineage. We found that ASCs cultured with ATRA or transfected with miRNA let-7e expressed epithelial markers such as cytokeratin-18 and early renal organogenesis markers such as Pax2, Wt1, Wnt4 and megalin. Conversely, the specific knockdown of miRNA let-7e in ASCs significantly decreased the expression of these genes, indicating its vital role during the differentiation process. Using luciferase reporter assays, we also showed that MMP9 is a direct target of miRNA let-7e. Thus, our results suggest that miRNA let-7e acts as a matrix metalloproteinase-9 (MMP9) inhibitor and differentiation inducer in ASCs.

Matèries

Obesitat, Citologia, Enzimologia, Genètica, Cèl·lules mare

Matèries (anglès)

Obesity, Cytology, Enzymology, Genetics, Stem cells

Citació

Col·leccions

Articles publicats en revistes (Ciències Clíniques)
Articles publicats en revistes (IDIBAPS: Institut d'investigacions Biomèdiques August Pi i Sunyer)

Citació

VENTAYOL ESPINAZO, Marina, VIÑAS MUÑOZ, José luis, SOLA MARTÍNEZ, Anna, JUNG, Michaela, BRÜNE, Bernhard, PI I SIQUÉS, Felip, MASTORA, Chrysoula, HOTTER CORRIPIO, Georgina. miRNA let-7e targeting MMP9 is involved in adipose-derived stem cell differentiation toward epithelia. _Cell Death and Disease_. 2014. Vol. 5, núm. e1048. [consulta: 26 de gener de 2026]. ISSN: 2041-4889. [Disponible a: https://hdl.handle.net/2445/133452]

Exportar metadades

JSON - METS

Compartir registre