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2017-12-13

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cc-by (c) Sanmartí Espinal, Marta et al., 2017
Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/126223

Quantification of interacting cognate odorants with olfactory receptors in nanovesicles

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https://doi.org/10.1038/s41598-017-16997-9

Abstract

This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors.

Subject

Olfacte, Biosensors, Olors, Bioenginyeria

Subject (English)

Smell, Biosensors, Odors, Bioengineering

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Articles publicats en revistes (Enginyeria Electrònica i Biomèdica)

Citation

SANMARTÍ ESPINAL, Marta, et al. Quantification of interacting cognate odorants with olfactory receptors in nanovesicles. Scientific Reports. 2017. Vol. 7, num. 17483. ISSN 2045-2322. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/126223

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