Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió publicadaData de publicació
Llicència de publicació
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/126223
Quantification of interacting cognate odorants with olfactory receptors in nanovesicles
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
This study aims to improve our understanding of the interaction between olfactory receptors and odorants to develop highly selective biosensing devices. Natural nanovesicles (NVs) from Saccharomyces cerevisiae, ~100 nm in diameter, carrying either the human OR17-40 or the chimpanzee OR7D4 olfactory receptor (OR) tagged with the c-myc epitope at their N-terminus, are presented as model systems to quantify the interaction between odorant and olfactory receptors. The level of expression of olfactory receptors was determined at individual NVs using a novel competitive ELISA immunoassay comparing the values obtained against those from techniques involving the solubilization of cell membrane proteins and the identification of c-myc-carrying receptors. Surface Plasmon Resonance (SPR) measurements on L1 Biacore chips indicate that cognate odorants bind to their Ors, thereby quantifying the approximate number of odorants that interact with a given olfactory receptor. The selectivity of OR17-40-carrying NVs towards helional and OR7D4-carrying NVs towards androstenone has been proven in cross-check experiments with non-specific odorant molecules (heptanal and pentadecalactone, respectively) and in control receptors.
Matèries
Matèries (anglès)
Citació
Citació
SANMARTÍ ESPINAL, Marta, IAVICOLI, Patrizia, CALÒ, Annalisa, TAULÉS I MARÍN, Marta, GALVE BOSCH, Roger, MARCO COLÁS, Ma. pilar, SAMITIER I MARTÍ, Josep. Quantification of interacting cognate odorants with olfactory receptors in nanovesicles. _Scientific Reports_. 2017. Vol. 7, núm. 17483. [consulta: 23 de gener de 2026]. ISSN: 2045-2322. [Disponible a: https://hdl.handle.net/2445/126223]