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NatCommun_Nadal_Lieb_et al_2025.pdf (10.63 MB)

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2025-03-17

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cc-by (c) Nadal Ribelles, Mariona et al., 2025
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/219972

Transcriptional heterogeneity shapes stress-adaptive responses in yeast

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Recurs relacionat

https://doi.org/10.1038/s41467-025-57911-6

Resum

In response to stress, cells activate signaling pathways that coordinate broad changes in gene expression to enhance cell survival. Remarkably, complex variations in gene expression occur even in isogenic populations and in response to similar signaling inputs. However, the molecular mechanisms underlying this variability and their influence on adaptive cell fate decisions are not fully understood. Here, we use scRNA-seq to longitudinally assess transcriptional dynamics during osmoadaptation in yeast. Our findings reveal highly heterogeneous expression of the osmoresponsive program, which organizes into combinatorial patterns that generate distinct cellular programs. The induction of these programs is favored by global transcriptome repression upon stress. Cells displaying basal expression of the osmoresponsive program are hyper-responsive and resistant to stress. Through a transcription-focused analysis of more than 300 RNA-barcoded deletion mutants, we identify genetic factors that shape the heterogeneity of the osmostress-induced transcriptome, define regulators of stress-related subpopulations and find a link between transcriptional heterogeneity and increased cell fitness. Our findings provide a regulatory map of the complex transcriptional phenotypes underlying osmoadaptation in yeast and highlight the importance of transcriptional heterogeneity in generating distinct adaptive strategies.

Matèries

Estrès (Fisiologia), Transcripció genètica

Matèries (anglès)

Stress (Physiology), Genetic transcription

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Col·leccions

Articles publicats en revistes (Institut de Recerca Biomèdica (IRB Barcelona))

Citació

NADAL RIBELLES, Mariona, et al. Transcriptional heterogeneity shapes stress-adaptive responses in yeast. Nature Communications. 2025. Vol. 16. ISSN 2041-1723. [consulted: 3 of August of 2026]. Available at: https://hdl.handle.net/2445/219972

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