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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/196932
Development and characterization of solid lipid nanoparticles (SLN) as a vehicle of biomolecules
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[eng] The interest of this work was to manufacture solid lipid nanoparticles that serve as a vehicle for biomolecules, specifically Chondroitin sulfate and pEGFP plasmid DNA. In the experimental part, the reagents and equipment used, and the methodology applied for the production and characterization of the nanoparticles are exposed, in accordance with the latest scientific trends contrasted bibliographically.
The nanoparticles were manufactured by the hot Microemulsion method, and the final formula was optimized by means of a 3x3x2 Experimental Factorial Design, where the critical operating parameters Concentration (mg/ml), Stirring rate (rpm) and Reaction Time were evaluated. (min).
Five valid formulas to encapsulate CHON demonstrated stability both nanoparticles in aqueous media and lyophilized nanoparticles. The physicochemical characterization of the formulations as CHON vehicle presented sizes smaller than 200nm, negative zeta potential and mainly circular morphology. At the level of biomolecular studies, the nanoparticles did not show cellular toxicity, they demonstrated the ability to internalize different cell lines and the ability to modify the inflammatory activity in inflamed cells. The ex vivo studies support the possibility of applying the nanoparticles as a CHON vehicle to be administered in a living being.
On the other hand, two nanoparticle formulations also proved to be useful as pDNA vehicles, presenting sizes smaller than 170 nm, positive zeta potential, and protecting DNA against enzymes that degrade this nucleic acid. The lipoplexes, formed between the nanoparticles and the DNA, did not show cytotoxicity and were able to transfect cells through the expression of the green fluorescent protein (EGFP).
With the study carried out, it will be possible in the future to propose said nanoparticles to treat certain diseases or that are useful in other applications in the pharmaceutical field.
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BUSTOS ARAYA, Marta eduviges. Development and characterization of solid lipid nanoparticles (SLN) as a vehicle of biomolecules. [consulta: 5 de desembre de 2025]. [Disponible a: https://hdl.handle.net/2445/196932]