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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/185442
High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting
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Objectives: To characterize the clonal complexity in Mycobacterium tuberculosis (MTB) infections considering factors that help maximize the detection of coexisting strains/variants. Methods: Genotypic analysis by Mycobacterial Interspersed Repetitive-Unit-Variable-Number Tandem-Repeats (MIRU-VNTR) was performed directly on 70 biopsy specimens from two or more different tissues involving 28 tuberculosis cases diagnosed post-mortem in Mozambique, a country with a high tuberculosis burden. Results: Genotypic data from isolates collected from two or more tissues were obtained for 23 of the 28 cases (82.1%), allowing the analysis of within-patient diversity. MIRU-VNTR analysis revealed clonal diversity in ten cases (35.7%). Five cases showed allelic differences in three or more loci, suggesting mixed infection with two different strains. In half of the cases showing within-host diversity, one of the specimens associated with clonal heterogeneity was brain tissue. Conclusions: Direct MTB genotyping from post-mortem tissue samples revealed a frequent within-host Mycobacterium tuberculosis diversity, including mixed and polyclonal infections. Most of this diversity would have been overlooked if only standard analysis of respiratory specimens had been performed.
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RODRÍGUEZ GRANDE, Cristina, et al. High within-host diversity found from direct genotyping on post-mortem tuberculosis specimens in a high-burden setting. Clinical Microbiology and Infection. 2021. Vol. 27, num. 10, pags. 1518. ISSN 1198-743X. [consulted: 11 of June of 2026]. Available at: https://hdl.handle.net/2445/185442