Carregant...
Fitxers
Tipus de document
ArticleVersió
Versió acceptadaData de publicació
Tots els drets reservats
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/162211
Novel instrument for automated pKa determination by internal standard capillary electrophoresis
Títol de la revista
Director/Tutor
ISSN de la revista
Títol del volum
Recurs relacionat
Resum
The internal standard capillary electrophoresis method (IS-CE) has been implemented in a novel sequential injection−capillary electrophoresis instrument for the high-throughput determination of acidity constants (pKa) regardless of aqueous solubility, number of pKa values, or structure. This instrument comprises a buffer creation system that automatically mixes within a few seconds four reagents for in situ creation of the separation electrolyte with a pH range of 2−13, ionic strength of 10−100 mM and organic solvent content from 0% to 40%. Combined with 1.2 kV/cm and a short effective length (15 cm to the UV detector) fast 20 s electrophoretic separations can be obtained. The low standard deviation of the replicates and the low variation compared to reference values show that this system can accurately determine acidity constants of drugs by IS-CE. A single pKa can be determined in 2 min and a set of 20 measurements in half an hour, allowing rapid, simple, and flexible determination of pKa values of pharmaceutical targets.
Matèries
Matèries (anglès)
Citació
Citació
CABOT CANYELLES, Joan marc, FUGUET I JORDÀ, Elisabet, ROSÉS PASCUAL, Martí, SMEJKAL, Petr, BREADMORE, Michael c.. Novel instrument for automated pKa determination by internal standard capillary electrophoresis. _Analytical Chemistry_. 2015. Vol. 87, núm. 12, pàgs. 6165-6172. [consulta: 20 de gener de 2026]. ISSN: 0003-2700. [Disponible a: https://hdl.handle.net/2445/162211]