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Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/66291
Mercury (II) and methylmercury determination in waters by liquid chromatography hyphenated to cold vapour atomic fluorescence spectrometry after online short-column preconcentration
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This paper reports a method developed for the simultaneous determination of methylmercury (MeHg+) and mercury(II) (Hg2+) species in water by liquid chromatography coupled to online UV irradiation and cold vapour atomic fluorescence spectrometry (LC-UV-CV-AFS) after online short-column preconcentration. This work focused on systematic studies of several variables to establish the maximum species recoveries, preconcentration factors and good reproducibility. The optimum results obtained were the following: 0.07 mmol L 1 2-mercaptoethanol as a complexing agent, precolumn conditioning with the mobile phase: a mixture of 80% of methanol (MeOH) and 20% of the following buffer: 0.0015 mol L 1 ammonium pyrrolidine dithiocarbamate (APDC) and 0.01 mol L 1 ammonium acetate (NH4CH3COO) at pH 5.5, 2 cm precolumn length and 2 mL min 1 sample flow. This method was applied to three water samples with different mineralisation contents. Various tests, based on spikes, were performed on each sample. A breakthrough volume of 4 mL was found. The recovery values of 72 3% and 81 5% for MeHg+ and Hg2+, respectively, were obtained regardless of the matrix composition, and the PF values were 30 and 32 for MeHg+ and Hg2+, respectively. The accuracy of the preconcentration method was verified by analysing a certified reference material. The detection limits (LDs) obtained were 15 ng L 1 for MeHg+ and 2 ng L 1 for Hg2+. The quantification limits (LQs) were 50 ng L 1 for both species. The established analytical online preconcentration method is suitable for the quantification of mercury species in a wide range of environmental waters.
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CARNEADO MORENO, Sergio, PERÓ GASCÓN, Roger, IBÁÑEZ PALOMINO, Carmen, LÓPEZ SÁNCHEZ, José fermín, SAHUQUILLO ESTRUGO, Àngels. Mercury (II) and methylmercury determination in waters by liquid chromatography hyphenated to cold vapour atomic fluorescence spectrometry after online short-column preconcentration. _Analytical Methods_. 2015. Vol. 7, núm. 2699-2706. [consulta: 29 de novembre de 2025]. ISSN: 1759-9660. [Disponible a: https://hdl.handle.net/2445/66291]