Miniatura

Fitxers

840095.pdf (7.21 MB)

Tipus de document

Article

Versió

Versió publicada

Data de publicació

2023

Llicència de publicació

cc-by-nc-nd (c) Noelia G. Romero, et al., 2023
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/219030

Developmental neurotoxicity evaluation of three Chinese herbal medicines in zebrafish larvae by means of two behavioral assays: Touch-evoked response and light/dark transition  

Títol de la revista

Autors

Director/Tutor

ISSN de la revista

Títol del volum

Recurs relacionat

https://doi.org/10.1016/j.reprotox.2023.108469

Resum

Chinese herbal medicine (CHM) is used among pregnant women. However, the question of its safety during pregnancy remains unclear. The use of these products relies on history of use data but there are specific toxicities like developmental neurotoxicity that are clearly understudied. Here we use the zebfrafish embryo developmental toxicity assay (ZEDTA) in combination with two behavioral assays: touch-evoked response and Light/Dark (L/D) transition assay to evaluate the neuro/developmental toxicity of three herbal products commonly used in CHM [Chinese name (abbreviation; part of the plant and Scientific name]: tian ma (TM; tuber form Gastrodia elata Blume), lei gong teng (LGT; root and rhizome of Tripterygium wilfordii Hook.f) and cha ye (green tea, leaves from Camellia sinensis (L.) Kuntze). In case significant alterations were detected, single components with potential exposure during pregnancy were identified in the literature and further tested. TM had no neurodevelopmental toxic potential in zebrafish embryos, while LGT and its main compounds triptolide and celastrol induced significant alterations in behavior. Developmental exposure to EGCG, the main catechin of green tea, also produced significant alterations in zebrafish embryos behavior after developmental exposure. A combination of ZEDTA with L/D Transition assay is proposed as a useful combination of alternative methods for DNT assessment of CHM products together with other New Approach Methodologies (NAMs). Keywords: Alternative methods; Herbal products; NAMs (New Approach Methodologies); Pregnancy; Prenatal development; Traditional use.

Matèries

Peix zebra, Embaràs, Toxicologia

Matèries (anglès)

Zebra danio, Pregnancy, Toxicology

Citació

Col·leccions

Articles publicats en revistes (Farmacologia, Toxicologia i Química Terapèutica)

Citació

ROMERO, Noelia giselle, GUTIERREZ, Gerardo, TEIXIDÓ CONDOMINES, Elisabet, LI, Lu, KLOSE, Jördis, PING CHUNG, Leung, CAÑIGUERAL I FOLCARÀ, Salvador, FRITSCHE, Ellen, BARENYS ESPADALER, Marta. Developmental neurotoxicity evaluation of three Chinese herbal medicines in zebrafish larvae by means of two behavioral assays: Touch-evoked response and light/dark transition  . _Reproductive Toxicology_. 2023. Vol. 121, núm. 108469. [consulta: 20 de gener de 2026]. ISSN: 0890-6238. [Disponible a: https://hdl.handle.net/2445/219030]

Exportar metadades

JSON - METS

Compartir registre