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2012-08-21

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cc-by (c) Perez Gil, Jordi et al., 2012
Si us plau utilitzeu sempre aquest identificador per citar o enllaçar aquest document: https://hdl.handle.net/2445/127789

Mutations in escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway

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Recurs relacionat

https://doi.org/10.1371/journal.pone.0043775

Resum

A functional 2-C-methyl-D-erythritol 4-phosphate (MEP) pathway is required for isoprenoid biosynthesis and hence survival in Escherichia coli and most other bacteria. In the first two steps of the pathway, MEP is produced from the central metabolic intermediates pyruvate and glyceraldehyde 3-phosphate via 1-deoxy-D-xylulose 5-phosphate (DXP) by the activity of the enzymes DXP synthase (DXS) and DXP reductoisomerase (DXR). Because the MEP pathway is absent from humans, it was proposed as a promising new target to develop new antibiotics. However, the lethal phenotype caused by the deletion of DXS or DXR was found to be suppressed with a relatively high efficiency by unidentified mutations. Here we report that several mutations in the unrelated genes aceE and ribB rescue growth of DXS-defective mutants because the encoded enzymes allowed the production of sufficient DXP in vivo. Together, this work unveils the diversity of mechanisms that can evolve in bacteria to circumvent a blockage of the first step of the MEP pathway.

Matèries

Escheríchia coli, Genètica, Metabolisme, Fisiologia, Proteïnes, Mutació (Biologia), Transferases

Matèries (anglès)

Escherichia coli, Genetics, Metabolism, Physiology, Proteins, Mutation (Biology), Transferases

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Articles publicats en revistes (Bioquímica i Biomedicina Molecular)

Citació

PEREZ GIL, Jordi, UROS, Eva maria, SAURET-GÜETO, Susanna, LOIS ROJAS, Luisa maría, KIRBY, James, NISHIMOTO, Minobu, BAIDOO, Edward e.k., KEASLING, Jay d., BORONAT I MARGOSA, Albert, RODRÍGUEZ CONCEPCIÓN, Manuel. Mutations in escherichia coli aceE and ribB genes allow survival of strains defective in the first step of the isoprenoid biosynthesis pathway. _PLoS One_. 2012. Vol. 7, núm. 8, pàgs. e43775-. [consulta: 23 de gener de 2026]. ISSN: 1932-6203. [Disponible a: https://hdl.handle.net/2445/127789]

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