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1993-10-25

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Please use this identifier to cite or link to this item: https://hdl.handle.net/2445/177551

Regulation of hepatic 6-phosphofructo-2-kinase/fructose 2,6-biphosphatase gene expression by glucagon

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Abstract

The control of hepatic 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene expression by glucagon was studied. Intraperitoneal administration of glucagon rapidly decreased the fructose 2,6-bisphosphate content by phosphorylation of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase and diminution of its Vmax. Immunologic studies using a specific liver antibody showed that the amount of enzyme rapidly decreased. Northern blot analysis showed that the isozyme expressed is the adult liver form. The 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase mRNA content decreased, whereas that of phosphoenolpyruvate carboxykinase increased, and that of albumin did not change. Run-on transcription assays with isolated nuclei showed inhibition in the relative transcription rate of the 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene and a stimulation of phosphoenolpyruvate carboxykinase gene. The regulation of mRNA stability of 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase by glucagon was also studied. The half-life of mRNA decreased in the presence of glucagon, suggesting that proteins modulated by a glucagon-dependent process are regulating its stability. The time course of mRNA levels correlated with the transcription inhibition of gene and destabilization of mRNA, indicating that glucagon modulates 6-phosphofructo-2-kinase/fructose 2,6-bisphosphatase gene expression at both transcriptional and posttranscriptional levels.

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Expressió gènica, Glucagó, Fetge, Fosforilació

Subject (English)

Gene expression, Glucagon, Liver, Phosphorylation

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Articles publicats en revistes (Ciències Fisiològiques)
Articles publicats en revistes (Bioquímica i Fisiologia)

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ROSA LÓPEZ, José Luis, et al. Regulation of hepatic 6-phosphofructo-2-kinase/fructose 2,6-biphosphatase gene expression by glucagon. Journal of Biological Chemistry. 1993. Vol. 268, num. 30, pags. 22540-22545. ISSN 0021-9258. [consulted: 15 of August of 2026]. Available at: https://hdl.handle.net/2445/177551

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